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Estimating infection prevalence: Best practices and their theoretical underpinnings.

Publication ,  Journal Article
Miller, IF; Schneider-Crease, I; Nunn, CL; Muehlenbein, MP
Published in: Ecology and evolution
July 2018

Accurately estimating infection prevalence is fundamental to the study of population health, disease dynamics, and infection risk factors. Prevalence is estimated as the proportion of infected individuals ("individual-based estimation"), but is also estimated as the proportion of samples in which evidence of infection is detected ("anonymous estimation"). The latter method is often used when researchers lack information on individual host identity, which can occur during noninvasive sampling of wild populations or when the individual that produced a fecal sample is unknown. The goal of this study was to investigate biases in individual-based versus anonymous prevalence estimation theoretically and to test whether mathematically derived predictions are evident in a comparative dataset of gastrointestinal helminth infections in nonhuman primates. Using a mathematical model, we predict that anonymous estimates of prevalence will be lower than individual-based estimates when (a) samples from infected individuals do not always contain evidence of infection and/or (b) when false negatives occur. The mathematical model further predicts that no difference in bias should exist between anonymous estimation and individual-based estimation when one sample is collected from each individual. Using data on helminth parasites of primates, we find that anonymous estimates of prevalence are significantly and substantially (12.17%) lower than individual-based estimates of prevalence. We also observed that individual-based estimates of prevalence from studies employing single sampling are on average 6.4% higher than anonymous estimates, suggesting a bias toward sampling infected individuals. We recommend that researchers use individual-based study designs with repeated sampling of individuals to obtain the most accurate estimate of infection prevalence. Moreover, to ensure accurate interpretation of their results and to allow for prevalence estimates to be compared among studies, it is essential that authors explicitly describe their sampling designs and prevalence calculations in publications.

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Published In

Ecology and evolution

DOI

EISSN

2045-7758

ISSN

2045-7758

Publication Date

July 2018

Volume

8

Issue

13

Start / End Page

6738 / 6747

Related Subject Headings

  • 4102 Ecological applications
  • 3104 Evolutionary biology
  • 3103 Ecology
  • 0603 Evolutionary Biology
  • 0602 Ecology
 

Citation

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MLA
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Miller, I. F., Schneider-Crease, I., Nunn, C. L., & Muehlenbein, M. P. (2018). Estimating infection prevalence: Best practices and their theoretical underpinnings. Ecology and Evolution, 8(13), 6738–6747. https://doi.org/10.1002/ece3.4179
Miller, Ian F., India Schneider-Crease, Charles L. Nunn, and Michael P. Muehlenbein. “Estimating infection prevalence: Best practices and their theoretical underpinnings.Ecology and Evolution 8, no. 13 (July 2018): 6738–47. https://doi.org/10.1002/ece3.4179.
Miller IF, Schneider-Crease I, Nunn CL, Muehlenbein MP. Estimating infection prevalence: Best practices and their theoretical underpinnings. Ecology and evolution. 2018 Jul;8(13):6738–47.
Miller, Ian F., et al. “Estimating infection prevalence: Best practices and their theoretical underpinnings.Ecology and Evolution, vol. 8, no. 13, July 2018, pp. 6738–47. Epmc, doi:10.1002/ece3.4179.
Miller IF, Schneider-Crease I, Nunn CL, Muehlenbein MP. Estimating infection prevalence: Best practices and their theoretical underpinnings. Ecology and evolution. 2018 Jul;8(13):6738–6747.
Journal cover image

Published In

Ecology and evolution

DOI

EISSN

2045-7758

ISSN

2045-7758

Publication Date

July 2018

Volume

8

Issue

13

Start / End Page

6738 / 6747

Related Subject Headings

  • 4102 Ecological applications
  • 3104 Evolutionary biology
  • 3103 Ecology
  • 0603 Evolutionary Biology
  • 0602 Ecology