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Joint Fluid Proteome after Anterior Cruciate Ligament Rupture Reflects an Acute Posttraumatic Inflammatory and Chondrodegenerative State.

Publication ,  Journal Article
King, JD; Rowland, G; Villasante Tezanos, AG; Warwick, J; Kraus, VB; Lattermann, C; Jacobs, CA
Published in: Cartilage
July 2020

OBJECTIVE: The purpose of this study was to evaluate changes in the synovial fluid proteome following acute anterior cruciate ligament (ACL) injury. DESIGN: This study represents a secondary analysis of synovial fluid samples collected from the placebo group of a previous randomized trial. Arthrocentesis was performed twice on 6 patients with an isolated acute ACL tear at a mean of 6 and 14 days postinjury. Synovial fluid was analyzed by a highly multiplexed assay of 1129 proteins (SOMAscan version 3, SomaLogic, Inc., Boulder, CO). Pathway analysis using DAVID was performed; genes included met 3 criteria: significant change between the 2 study time points using a paired t test, significant change between the 2 study time points using a Mann-Whitney nonparametric test, and significant Benjamini post hoc analysis. RESULTS: Fifteen analytes demonstrated significant increases between time points. Five of the 15 have been previously associated with the onset and/or severity of rheumatoid arthritis, including apoliopoprotein E and isoform E3, vascular cell adhesion protein 1, interleukin-34, and cell surface glycoprotein CD200 receptor 1. Chondrodegenerative enzymes and products of cartilage degeneration all increased over time following injury: MMP-1 (P = 0.08, standardized response mean [SRM] = 1.00), MMP-3 (P = 0.05, SRM = 0.90), ADAM12 (P = 0.03, SRM = 1.31), aggrecan (P = 0.08, SRM = 1.13), and CTX-II (P = 0.07, SRM = 0.56). Notable pathways that were differentially expressed following injury were the cytokine-cytokine receptor interaction and osteoclast differentiation pathways. CONCLUSIONS: The proteomic results and pathway analysis demonstrated a pattern of cartilage degeneration, not only consistent with previous findings but also changes consistent with an inflammatory arthritogenic process post-ACL injury.

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Published In

Cartilage

DOI

EISSN

1947-6043

Publication Date

July 2020

Volume

11

Issue

3

Start / End Page

329 / 337

Location

United States

Related Subject Headings

  • Young Adult
  • Synovial Fluid
  • Signal Transduction
  • Rupture
  • Randomized Controlled Trials as Topic
  • Proteomics
  • Proteome
  • Osteoarthritis, Knee
  • Male
  • Knee Joint
 

Citation

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Chicago
ICMJE
MLA
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King, J. D., Rowland, G., Villasante Tezanos, A. G., Warwick, J., Kraus, V. B., Lattermann, C., & Jacobs, C. A. (2020). Joint Fluid Proteome after Anterior Cruciate Ligament Rupture Reflects an Acute Posttraumatic Inflammatory and Chondrodegenerative State. Cartilage, 11(3), 329–337. https://doi.org/10.1177/1947603518790009
King, John D., Grant Rowland, Alejandro G. Villasante Tezanos, James Warwick, Virginia B. Kraus, Christian Lattermann, and Cale A. Jacobs. “Joint Fluid Proteome after Anterior Cruciate Ligament Rupture Reflects an Acute Posttraumatic Inflammatory and Chondrodegenerative State.Cartilage 11, no. 3 (July 2020): 329–37. https://doi.org/10.1177/1947603518790009.
King JD, Rowland G, Villasante Tezanos AG, Warwick J, Kraus VB, Lattermann C, et al. Joint Fluid Proteome after Anterior Cruciate Ligament Rupture Reflects an Acute Posttraumatic Inflammatory and Chondrodegenerative State. Cartilage. 2020 Jul;11(3):329–37.
King, John D., et al. “Joint Fluid Proteome after Anterior Cruciate Ligament Rupture Reflects an Acute Posttraumatic Inflammatory and Chondrodegenerative State.Cartilage, vol. 11, no. 3, July 2020, pp. 329–37. Pubmed, doi:10.1177/1947603518790009.
King JD, Rowland G, Villasante Tezanos AG, Warwick J, Kraus VB, Lattermann C, Jacobs CA. Joint Fluid Proteome after Anterior Cruciate Ligament Rupture Reflects an Acute Posttraumatic Inflammatory and Chondrodegenerative State. Cartilage. 2020 Jul;11(3):329–337.
Journal cover image

Published In

Cartilage

DOI

EISSN

1947-6043

Publication Date

July 2020

Volume

11

Issue

3

Start / End Page

329 / 337

Location

United States

Related Subject Headings

  • Young Adult
  • Synovial Fluid
  • Signal Transduction
  • Rupture
  • Randomized Controlled Trials as Topic
  • Proteomics
  • Proteome
  • Osteoarthritis, Knee
  • Male
  • Knee Joint