Skip to main content

Antiviral Effects of ABMA against Herpes Simplex Virus Type 2 In Vitro and In Vivo.

Publication ,  Journal Article
Dai, W; Wu, Y; Bi, J; Wang, S; Li, F; Kong, W; Barbier, J; Cintrat, J-C; Gao, F; Gillet, D; Su, W; Jiang, C
Published in: Viruses
March 9, 2018

Herpes simplex virus type 2 (HSV-2) is the causative pathogen of genital herpes and is closely associated with the occurrence of cervical cancer and human immunodeficiency virus (HIV) infection. The absence of an effective vaccine and the emergence of drug resistance to commonly used nucleoside analogs emphasize the urgent need for alternative antivirals against HSV-2. Recently, ABMA [1-adamantyl (5-bromo-2-methoxybenzyl) amine] has been demonstrated to be an inhibitor of several pathogens exploiting host-vesicle transport, which also participates in the HSV-2 lifecycle. Here, we showed that ABMA inhibited HSV-2-induced cytopathic effects and plaque formation with 50% effective concentrations of 1.66 and 1.08 μM, respectively. We also preliminarily demonstrated in a time of compound addition assay that ABMA exerted a dual antiviral mechanism by impairing virus entry, as well as the late stages of the HSV-2 lifecycle. Furthermore, in vivo studies showed that ABMA protected BALB/c mice from intravaginal HSV-2 challenge with an improved survival rate of 50% at 5 mg/kg (8.33% for the untreated virus infected control). Consequently, our study has identified ABMA as an effective inhibitor of HSV-2, both in vitro and in vivo, for the first time and presents an alternative to nucleoside analogs for HSV-2 infection treatment.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Viruses

DOI

EISSN

1999-4915

Publication Date

March 9, 2018

Volume

10

Issue

3

Location

Switzerland

Related Subject Headings

  • Virus Replication
  • Virus Internalization
  • Viral Load
  • Vero Cells
  • Molecular Structure
  • Microbial Sensitivity Tests
  • Mice, Inbred BALB C
  • Mice
  • Herpesvirus 2, Human
  • Herpes Simplex
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Dai, W., Wu, Y., Bi, J., Wang, S., Li, F., Kong, W., … Jiang, C. (2018). Antiviral Effects of ABMA against Herpes Simplex Virus Type 2 In Vitro and In Vivo. Viruses, 10(3). https://doi.org/10.3390/v10030119
Dai, Wenwen, Yu Wu, Jinpeng Bi, Shuai Wang, Fang Li, Wei Kong, Julien Barbier, et al. “Antiviral Effects of ABMA against Herpes Simplex Virus Type 2 In Vitro and In Vivo.Viruses 10, no. 3 (March 9, 2018). https://doi.org/10.3390/v10030119.
Dai W, Wu Y, Bi J, Wang S, Li F, Kong W, et al. Antiviral Effects of ABMA against Herpes Simplex Virus Type 2 In Vitro and In Vivo. Viruses. 2018 Mar 9;10(3).
Dai, Wenwen, et al. “Antiviral Effects of ABMA against Herpes Simplex Virus Type 2 In Vitro and In Vivo.Viruses, vol. 10, no. 3, Mar. 2018. Pubmed, doi:10.3390/v10030119.
Dai W, Wu Y, Bi J, Wang S, Li F, Kong W, Barbier J, Cintrat J-C, Gao F, Gillet D, Su W, Jiang C. Antiviral Effects of ABMA against Herpes Simplex Virus Type 2 In Vitro and In Vivo. Viruses. 2018 Mar 9;10(3).

Published In

Viruses

DOI

EISSN

1999-4915

Publication Date

March 9, 2018

Volume

10

Issue

3

Location

Switzerland

Related Subject Headings

  • Virus Replication
  • Virus Internalization
  • Viral Load
  • Vero Cells
  • Molecular Structure
  • Microbial Sensitivity Tests
  • Mice, Inbred BALB C
  • Mice
  • Herpesvirus 2, Human
  • Herpes Simplex