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A new EV71 VP3 epitope in norovirus P particle vector displays neutralizing activity and protection in vivo in mice.

Publication ,  Journal Article
Jiang, L; Fan, R; Sun, S; Fan, P; Su, W; Zhou, Y; Gao, F; Xu, F; Kong, W; Jiang, C
Published in: Vaccine
November 27, 2015

Enterovirus 71 (EV71) and Coxsackievirus A16 (CVA16), as the main agents causing hand, foot and mouth disease (HFMD), have become a serious public health concern in the Asia-Pacific region. Recently, various neutralizing B cell epitopes of EV71 were identified as targets for promising vaccine candidates. Structural studies of Picornaviridae indicated that potent immunodominant epitopes typically lie in the hypervariable loop of capsid surfaces. However, cross-neutralizing antibodies and cross-protection between EV71 and CVA16 have not been observed. Therefore, we speculated that divergent sequences of the two viruses are key epitopes for inducing protective neutralizing responses. In this study, we selected 10 divergent epitope candidates based on alignment of the EV71 and CVA16 P1 amino acid sequences using the Multalin interface page, and these epitopes are conserved among all subgenotypes of EV71. Simultaneously, by utilizing the norovirus P particle as a novel vaccine delivery carrier, we identified the 71-6 epitope (amino acid 176-190 of VP3) as a conformational neutralizing epitope against EV71 in an in vitro micro-neutralization assay as well as an in vivo protection assay in mice. Altogether, these results indicated that the incorporation of the 71-6 epitope into the norovirus P domain can provide a promising candidate for an effective synthetic peptide-based vaccine against EV71.

Duke Scholars

Published In

Vaccine

DOI

EISSN

1873-2518

Publication Date

November 27, 2015

Volume

33

Issue

48

Start / End Page

6596 / 6603

Location

Netherlands

Related Subject Headings

  • Virology
  • Viral Vaccines
  • Vaccines, Virus-Like Particle
  • Vaccines, Subunit
  • Norovirus
  • Neutralization Tests
  • Mice
  • Epitopes, B-Lymphocyte
  • Enterovirus Infections
  • Enterovirus
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Jiang, L., Fan, R., Sun, S., Fan, P., Su, W., Zhou, Y., … Jiang, C. (2015). A new EV71 VP3 epitope in norovirus P particle vector displays neutralizing activity and protection in vivo in mice. Vaccine, 33(48), 6596–6603. https://doi.org/10.1016/j.vaccine.2015.10.104
Jiang, Liping, Rongjun Fan, Shiyang Sun, Peihu Fan, Weiheng Su, Yan Zhou, Feng Gao, Fei Xu, Wei Kong, and Chunlai Jiang. “A new EV71 VP3 epitope in norovirus P particle vector displays neutralizing activity and protection in vivo in mice.Vaccine 33, no. 48 (November 27, 2015): 6596–6603. https://doi.org/10.1016/j.vaccine.2015.10.104.
Jiang L, Fan R, Sun S, Fan P, Su W, Zhou Y, et al. A new EV71 VP3 epitope in norovirus P particle vector displays neutralizing activity and protection in vivo in mice. Vaccine. 2015 Nov 27;33(48):6596–603.
Jiang, Liping, et al. “A new EV71 VP3 epitope in norovirus P particle vector displays neutralizing activity and protection in vivo in mice.Vaccine, vol. 33, no. 48, Nov. 2015, pp. 6596–603. Pubmed, doi:10.1016/j.vaccine.2015.10.104.
Jiang L, Fan R, Sun S, Fan P, Su W, Zhou Y, Gao F, Xu F, Kong W, Jiang C. A new EV71 VP3 epitope in norovirus P particle vector displays neutralizing activity and protection in vivo in mice. Vaccine. 2015 Nov 27;33(48):6596–6603.
Journal cover image

Published In

Vaccine

DOI

EISSN

1873-2518

Publication Date

November 27, 2015

Volume

33

Issue

48

Start / End Page

6596 / 6603

Location

Netherlands

Related Subject Headings

  • Virology
  • Viral Vaccines
  • Vaccines, Virus-Like Particle
  • Vaccines, Subunit
  • Norovirus
  • Neutralization Tests
  • Mice
  • Epitopes, B-Lymphocyte
  • Enterovirus Infections
  • Enterovirus