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Utility of peripheral blood dual color, double fusion fluorescent in situ hybridization for BCR/ABL fusion to assess cytogenetic remission status in chronic myeloid leukemia.

Publication ,  Journal Article
Landstrom, AP; Ketterling, RP; Knudson, RA; Tefferi, A
Published in: Leuk Lymphoma
October 2006

The molecular signature of BCR-ABL fusion in chronic myeloid leukemia (CML) provides a unique tool for diagnosis and monitoring of tumor burden during therapy. The gold standard in this regard is conventional bone marrow cytogenetics. Peripheral blood fluorescent in situ hybridization (FISH) offers the possibility of a less invasive, more practical alternative method in terms of both cost and turnaround time. In the present study, we examined 296 paired samples from 65 patients with CML and demonstrate a tight correlation, in quantifying BCR-ABL burden between bone marrow cytogenetics, bone marrow dual color, double fusion (D-FISH), and peripheral blood D-FISH (P < 0.0001 for each). Furthermore, we demonstrate that peripheral blood D-FISH can be used as a surrogate for cytogenetic studies in monitoring cytogenetic remission status.

Duke Scholars

Published In

Leuk Lymphoma

DOI

ISSN

1042-8194

Publication Date

October 2006

Volume

47

Issue

10

Start / End Page

2055 / 2061

Location

United States

Related Subject Headings

  • Remission Induction
  • Regression Analysis
  • Philadelphia Chromosome
  • Middle Aged
  • Leukemia, Myelogenous, Chronic, BCR-ABL Positive
  • In Situ Hybridization, Fluorescence
  • Immunology
  • Humans
  • Fusion Proteins, bcr-abl
  • Cytogenetics
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Landstrom, A. P., Ketterling, R. P., Knudson, R. A., & Tefferi, A. (2006). Utility of peripheral blood dual color, double fusion fluorescent in situ hybridization for BCR/ABL fusion to assess cytogenetic remission status in chronic myeloid leukemia. Leuk Lymphoma, 47(10), 2055–2061. https://doi.org/10.1080/10428190600783551
Landstrom, Andrew P., Rhett P. Ketterling, Ryan A. Knudson, and Ayalew Tefferi. “Utility of peripheral blood dual color, double fusion fluorescent in situ hybridization for BCR/ABL fusion to assess cytogenetic remission status in chronic myeloid leukemia.Leuk Lymphoma 47, no. 10 (October 2006): 2055–61. https://doi.org/10.1080/10428190600783551.
Landstrom, Andrew P., et al. “Utility of peripheral blood dual color, double fusion fluorescent in situ hybridization for BCR/ABL fusion to assess cytogenetic remission status in chronic myeloid leukemia.Leuk Lymphoma, vol. 47, no. 10, Oct. 2006, pp. 2055–61. Pubmed, doi:10.1080/10428190600783551.
Journal cover image

Published In

Leuk Lymphoma

DOI

ISSN

1042-8194

Publication Date

October 2006

Volume

47

Issue

10

Start / End Page

2055 / 2061

Location

United States

Related Subject Headings

  • Remission Induction
  • Regression Analysis
  • Philadelphia Chromosome
  • Middle Aged
  • Leukemia, Myelogenous, Chronic, BCR-ABL Positive
  • In Situ Hybridization, Fluorescence
  • Immunology
  • Humans
  • Fusion Proteins, bcr-abl
  • Cytogenetics