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Performance of Homologous and Heterologous Prime-Boost Immunization Regimens of Recombinant Adenovirus and Modified Vaccinia Virus Ankara Expressing an Ag85B-TB10.4 Fusion Protein against Mycobacterium tuberculosis.

Publication ,  Journal Article
Kou, Y; Wan, M; Shi, W; Liu, J; Zhao, Z; Xu, Y; Wei, W; Sun, B; Gao, F; Cai, L; Jiang, C
Published in: J Microbiol Biotechnol
June 28, 2018

Tuberculosis (TB) remains a serious health issue around the word. Adenovirus (Ad)-based vaccine and modified vaccinia virus Ankara (MVA)-based vaccine have emerged as two of the most promising immunization candidates over the past few years. However, the performance of the homologous and heterologous prime-boost immunization regimens of these two viral vector-based vaccines remains unclear. In the present study, we constructed recombinant Ad and MVA expressing an Ag85B-TB10.4 fusion protein (AdH4 and MVAH4) and evaluated the impact of their different immunization regimens on the humoral and cellular immune responses. We found that the viral vector-based vaccines could generate significantly higher levels of antigen-specific antibodies, IFN-γ-producing splenocytes, CD69⁺CD8⁺ T cells, and IFN-γ secretion when compared with bacillus Calmette-Guérin (BCG) in a mouse model. AdH4-containing immunization regimens (AdH4-AdH4, AdH4-MVAH4, and MVAH4-AdH4) induced significantly stronger antibody responses, much more IFN-γ-producing splenocytes and CD69⁺CD8⁺ T cells, and higher levels of IFN-γ secretion when compared with the MVAH4-MVAH4 immunization regimen. The number of IFN-γ-producing splenocytes sensitive to CD8⁺ T-cell restricted peptides of Ag85B (9-1p and 9-2p) and Th1-related cytokines (IFN-γ and TNF-α) in the AdH4-MVAH4 heterologous prime-boost regimen immunization group was significantly higher than that in the other viral vector-based vaccine- and BCG-immunized groups, respectively. These results indicate that an immunization regimen involving AdH4 may have a higher capacity to induce humoral and cellular immune responses against TB in mice than that by regimens containing BCG or MVAH4 alone, and the AdH4-MVAH4 prime-boost regimen may generate an ideal protective effect.

Duke Scholars

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Published In

J Microbiol Biotechnol

DOI

EISSN

1738-8872

Publication Date

June 28, 2018

Volume

28

Issue

6

Start / End Page

1022 / 1029

Location

Korea (South)

Related Subject Headings

  • Vaccinia virus
  • Vaccines, Synthetic
  • Tuberculosis Vaccines
  • Tuberculosis
  • Treatment Outcome
  • Recombinant Fusion Proteins
  • Mycobacterium tuberculosis
  • Mice
  • Immunization
  • Immunity, Humoral
 

Citation

APA
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ICMJE
MLA
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Kou, Y., Wan, M., Shi, W., Liu, J., Zhao, Z., Xu, Y., … Jiang, C. (2018). Performance of Homologous and Heterologous Prime-Boost Immunization Regimens of Recombinant Adenovirus and Modified Vaccinia Virus Ankara Expressing an Ag85B-TB10.4 Fusion Protein against Mycobacterium tuberculosis. J Microbiol Biotechnol, 28(6), 1022–1029. https://doi.org/10.4014/jmb.1712.12064
Kou, Yiming, Mingming Wan, Wei Shi, Jie Liu, Zhilei Zhao, Yongqing Xu, Wei Wei, et al. “Performance of Homologous and Heterologous Prime-Boost Immunization Regimens of Recombinant Adenovirus and Modified Vaccinia Virus Ankara Expressing an Ag85B-TB10.4 Fusion Protein against Mycobacterium tuberculosis.J Microbiol Biotechnol 28, no. 6 (June 28, 2018): 1022–29. https://doi.org/10.4014/jmb.1712.12064.

Published In

J Microbiol Biotechnol

DOI

EISSN

1738-8872

Publication Date

June 28, 2018

Volume

28

Issue

6

Start / End Page

1022 / 1029

Location

Korea (South)

Related Subject Headings

  • Vaccinia virus
  • Vaccines, Synthetic
  • Tuberculosis Vaccines
  • Tuberculosis
  • Treatment Outcome
  • Recombinant Fusion Proteins
  • Mycobacterium tuberculosis
  • Mice
  • Immunization
  • Immunity, Humoral