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Identification of human and mouse p19, a novel CDK4 and CDK6 inhibitor with homology to p16ink4.

Publication ,  Journal Article
Chan, FK; Zhang, J; Cheng, L; Shapiro, DN; Winoto, A
Published in: Mol Cell Biol
May 1995

The cell cycle in mammalian cells is regulated by a series of cyclins and cyclin-dependent kinases (CDKs). The G1/S checkpoint is mainly dictated by the kinase activities of the cyclin D-CDK4 and/or cyclin D-CDK6 complex and the cyclin E-CDK2 complex. These G1 kinases can in turn be regulated by cell cycle inhibitors, which may cause the cells to arrest at the G1 phase. In T-cell hybridomas, addition of anti-T-cell receptor antibody results not only in G1 arrest but also in apoptosis. In searching for a protein(s) which might interact with Nur77, an orphan steroid receptor required for activation-induced apoptosis of T-cell hybridomas, we have cloned a novel human and mouse CDK inhibitor, p19. The deduced p19 amino acid sequence consists of four ankyrin repeats with 48% identity to p16. The human p19 gene is located on chromosome 19p13, distinct from the positions of p18, p16, and p15. Its mRNA is expressed in all cell types examined. The p19 fusion protein can associate in vitro with CDK4 but not with CDK2, CDC2, or cyclin A, B, E, or D1 to D3. Addition of p19 protein can lead to inhibition of the in vitro kinase activity of cyclin D-CDK4 but not that of cyclin E-CDK2. In T-cell hybridoma DO11.10, p19 was found in association with CDK4 and CDK6 in vivo, although its association with Nur77 is not clear at this point. Thus, p19 is a novel CDK inhibitor which may play a role in the cell cycle regulation of T cells.

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Published In

Mol Cell Biol

DOI

ISSN

0270-7306

Publication Date

May 1995

Volume

15

Issue

5

Start / End Page

2682 / 2688

Location

United States

Related Subject Headings

  • T-Lymphocytes
  • Sequence Homology, Amino Acid
  • Proto-Oncogene Proteins
  • Protein Serine-Threonine Kinases
  • Molecular Sequence Data
  • Mice
  • In Situ Hybridization, Fluorescence
  • Humans
  • Developmental Biology
  • Cyclin-Dependent Kinases
 

Citation

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Chan, F. K., Zhang, J., Cheng, L., Shapiro, D. N., & Winoto, A. (1995). Identification of human and mouse p19, a novel CDK4 and CDK6 inhibitor with homology to p16ink4. Mol Cell Biol, 15(5), 2682–2688. https://doi.org/10.1128/MCB.15.5.2682
Chan, F. K., J. Zhang, L. Cheng, D. N. Shapiro, and A. Winoto. “Identification of human and mouse p19, a novel CDK4 and CDK6 inhibitor with homology to p16ink4.Mol Cell Biol 15, no. 5 (May 1995): 2682–88. https://doi.org/10.1128/MCB.15.5.2682.
Chan FK, Zhang J, Cheng L, Shapiro DN, Winoto A. Identification of human and mouse p19, a novel CDK4 and CDK6 inhibitor with homology to p16ink4. Mol Cell Biol. 1995 May;15(5):2682–8.
Chan, F. K., et al. “Identification of human and mouse p19, a novel CDK4 and CDK6 inhibitor with homology to p16ink4.Mol Cell Biol, vol. 15, no. 5, May 1995, pp. 2682–88. Pubmed, doi:10.1128/MCB.15.5.2682.
Chan FK, Zhang J, Cheng L, Shapiro DN, Winoto A. Identification of human and mouse p19, a novel CDK4 and CDK6 inhibitor with homology to p16ink4. Mol Cell Biol. 1995 May;15(5):2682–2688.

Published In

Mol Cell Biol

DOI

ISSN

0270-7306

Publication Date

May 1995

Volume

15

Issue

5

Start / End Page

2682 / 2688

Location

United States

Related Subject Headings

  • T-Lymphocytes
  • Sequence Homology, Amino Acid
  • Proto-Oncogene Proteins
  • Protein Serine-Threonine Kinases
  • Molecular Sequence Data
  • Mice
  • In Situ Hybridization, Fluorescence
  • Humans
  • Developmental Biology
  • Cyclin-Dependent Kinases