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Metabolic glycan labeling and chemoselective functionalization of native biomaterials.

Publication ,  Journal Article
Ren, X; Evangelista-Leite, D; Wu, T; Rajab, TK; Moser, PT; Kitano, K; Economopoulos, KP; Gorman, DE; Bloom, JP; Tan, JJ; Gilpin, SE; Zhou, H ...
Published in: Biomaterials
November 2018

Decellularized native extracellular matrix (ECM) biomaterials are widely used in tissue engineering and have reached clinical application as biomesh implants. To enhance their regenerative properties and postimplantation performance, ECM biomaterials could be functionalized via immobilization of bioactive molecules. To facilitate ECM functionalization, we developed a metabolic glycan labeling approach using physiologic pathways to covalently incorporate click-reactive azide ligands into the native ECM of a wide variety of rodent tissues and organs in vivo, and into the ECM of isolated rodent and porcine lungs cultured ex vivo. The incorporated azides within the ECM were preserved after decellularization and served as chemoselective ligands for subsequent bioconjugation via click chemistry. As proof of principle, we generated alkyne-modified heparin, immobilized it onto azide-incorporated acellular lungs, and demonstrated its bioactivity by Antithrombin III immobilization and Factor Xa inhibition. The herein reported metabolic glycan labeling approach represents a novel platform technology for manufacturing click-reactive native ECM biomaterials, thereby enabling efficient and chemoselective functionalization of these materials to facilitate tissue regeneration and repair.

Duke Scholars

Published In

Biomaterials

DOI

EISSN

1878-5905

ISSN

0142-9612

Publication Date

November 2018

Volume

182

Start / End Page

127 / 134

Related Subject Headings

  • Tissue Scaffolds
  • Swine
  • Staining and Labeling
  • Rats, Sprague-Dawley
  • Rats
  • Polysaccharides
  • Male
  • Lung
  • Heparin
  • Extracellular Matrix
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Ren, X., Evangelista-Leite, D., Wu, T., Rajab, T. K., Moser, P. T., Kitano, K., … Ott, H. C. (2018). Metabolic glycan labeling and chemoselective functionalization of native biomaterials. Biomaterials, 182, 127–134. https://doi.org/10.1016/j.biomaterials.2018.08.012
Ren, Xi, Daniele Evangelista-Leite, Tong Wu, Taufiek K. Rajab, Philipp T. Moser, Kentaro Kitano, Konstantinos P. Economopoulos, et al. “Metabolic glycan labeling and chemoselective functionalization of native biomaterials.Biomaterials 182 (November 2018): 127–34. https://doi.org/10.1016/j.biomaterials.2018.08.012.
Ren X, Evangelista-Leite D, Wu T, Rajab TK, Moser PT, Kitano K, et al. Metabolic glycan labeling and chemoselective functionalization of native biomaterials. Biomaterials. 2018 Nov;182:127–34.
Ren, Xi, et al. “Metabolic glycan labeling and chemoselective functionalization of native biomaterials.Biomaterials, vol. 182, Nov. 2018, pp. 127–34. Epmc, doi:10.1016/j.biomaterials.2018.08.012.
Ren X, Evangelista-Leite D, Wu T, Rajab TK, Moser PT, Kitano K, Economopoulos KP, Gorman DE, Bloom JP, Tan JJ, Gilpin SE, Zhou H, Mathisen DJ, Ott HC. Metabolic glycan labeling and chemoselective functionalization of native biomaterials. Biomaterials. 2018 Nov;182:127–134.
Journal cover image

Published In

Biomaterials

DOI

EISSN

1878-5905

ISSN

0142-9612

Publication Date

November 2018

Volume

182

Start / End Page

127 / 134

Related Subject Headings

  • Tissue Scaffolds
  • Swine
  • Staining and Labeling
  • Rats, Sprague-Dawley
  • Rats
  • Polysaccharides
  • Male
  • Lung
  • Heparin
  • Extracellular Matrix