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High-throughput cell focusing and separation via acoustofluidic tweezers.

Publication ,  Journal Article
Wu, M; Chen, K; Yang, S; Wang, Z; Huang, P-H; Mai, J; Li, Z-Y; Huang, TJ
Published in: Lab on a chip
September 2018

Separation of particles and cells is an important function in many biological and biomedical protocols. Although a variety of microfluidic-based techniques have been developed so far, there is clearly still a demand for a precise, fast, and biocompatible method for separation of microparticles and cells. By combining acoustics and hydrodynamics, we have developed a method which we integrated into three-dimensional acoustofluidic tweezers (3D-AFT) to rapidly and efficiently separate microparticles and cells into multiple high-purity fractions. Compared with other acoustophoresis methods, this 3D-AFT method significantly increases the throughput by an order of magnitude, is label-free and gently handles the sorted cells. We demonstrate not only the separation of 10, 12, and 15 micron particles at a throughput up to 500 μl min-1 using this 3D-AFT method, but also the separation of erythrocytes, leukocytes, and cancer cells. This 3D-AFT method is able to meet various separation demands thus offering a viable alternative with potential for clinical applications.

Duke Scholars

Published In

Lab on a chip

DOI

EISSN

1473-0189

ISSN

1473-0197

Publication Date

September 2018

Volume

18

Issue

19

Start / End Page

3003 / 3010

Related Subject Headings

  • Particle Size
  • Leukocytes
  • Lab-On-A-Chip Devices
  • Humans
  • Erythrocytes
  • Cell Separation
  • Cell Line, Tumor
  • Analytical Chemistry
  • Acoustics
  • 40 Engineering
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Wu, M., Chen, K., Yang, S., Wang, Z., Huang, P.-H., Mai, J., … Huang, T. J. (2018). High-throughput cell focusing and separation via acoustofluidic tweezers. Lab on a Chip, 18(19), 3003–3010. https://doi.org/10.1039/c8lc00434j
Wu, Mengxi, Kejie Chen, Shujie Yang, Zeyu Wang, Po-Hsun Huang, John Mai, Zeng-Yao Li, and Tony Jun Huang. “High-throughput cell focusing and separation via acoustofluidic tweezers.Lab on a Chip 18, no. 19 (September 2018): 3003–10. https://doi.org/10.1039/c8lc00434j.
Wu M, Chen K, Yang S, Wang Z, Huang P-H, Mai J, et al. High-throughput cell focusing and separation via acoustofluidic tweezers. Lab on a chip. 2018 Sep;18(19):3003–10.
Wu, Mengxi, et al. “High-throughput cell focusing and separation via acoustofluidic tweezers.Lab on a Chip, vol. 18, no. 19, Sept. 2018, pp. 3003–10. Epmc, doi:10.1039/c8lc00434j.
Wu M, Chen K, Yang S, Wang Z, Huang P-H, Mai J, Li Z-Y, Huang TJ. High-throughput cell focusing and separation via acoustofluidic tweezers. Lab on a chip. 2018 Sep;18(19):3003–3010.
Journal cover image

Published In

Lab on a chip

DOI

EISSN

1473-0189

ISSN

1473-0197

Publication Date

September 2018

Volume

18

Issue

19

Start / End Page

3003 / 3010

Related Subject Headings

  • Particle Size
  • Leukocytes
  • Lab-On-A-Chip Devices
  • Humans
  • Erythrocytes
  • Cell Separation
  • Cell Line, Tumor
  • Analytical Chemistry
  • Acoustics
  • 40 Engineering