Routine single particle CryoEM sample and grid characterization by tomography.
Single particle cryo-electron microscopy (cryoEM) is often performed under the assumption that particles are not adsorbed to the air-water interfaces and in thin, vitreous ice. In this study, we performed fiducial-less tomography on over 50 different cryoEM grid/sample preparations to determine the particle distribution within the ice and the overall geometry of the ice in grid holes. Surprisingly, by studying particles in holes in 3D from over 1000 tomograms, we have determined that the vast majority of particles (approximately 90%) are adsorbed to an air-water interface. The implications of this observation are wide-ranging, with potential ramifications regarding protein denaturation, conformational change, and preferred orientation. We also show that fiducial-less cryo-electron tomography on single particle grids may be used to determine ice thickness, optimal single particle collection areas and strategies, particle heterogeneity, and de novo models for template picking and single particle alignment.
Duke Scholars
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- Water
- Surface Properties
- Sugar Alcohol Dehydrogenases
- Rabbits
- Proteasome Endopeptidase Complex
- Fructose-Bisphosphate Aldolase
- Escherichia coli
- Electron Microscope Tomography
- DnaB Helicases
- Cryoelectron Microscopy
Citation
Published In
DOI
EISSN
Publication Date
Volume
Location
Related Subject Headings
- Water
- Surface Properties
- Sugar Alcohol Dehydrogenases
- Rabbits
- Proteasome Endopeptidase Complex
- Fructose-Bisphosphate Aldolase
- Escherichia coli
- Electron Microscope Tomography
- DnaB Helicases
- Cryoelectron Microscopy