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IL-6 and TNF-alpha expression in, and release from, contracting human skeletal muscle.

Publication ,  Journal Article
Steensberg, A; Keller, C; Starkie, RL; Osada, T; Febbraio, MA; Pedersen, BK
Published in: Am J Physiol Endocrinol Metab
December 2002

The aim of the present study was to examine whether IL-6 and TNF-alpha are expressed in, and released from, human skeletal muscle during exercise. We hypothesized that the skeletal muscle will release IL-6, but not TNF-alpha, during exercise because of previous observations that TNF-alpha negatively affects glucose uptake in skeletal muscle. Six healthy, male subjects performed 180 min of two-legged knee-extensor exercise. Muscle samples were obtained from the vastus lateralis of one limb. In addition, blood samples were obtained from a femoral artery and vein. Plasma was analyzed for IL-6 and TNF-alpha. We detected both IL-6 and TNF-alpha mRNA in resting muscle samples, and whereas IL-6 increased (P < 0.05) approximately 100-fold throughout exercise, no significant increase in TNF-alpha mRNA was observed. Arterial plasma TNF-alpha did not increase during exercise. Furthermore, there was no net release of TNF-alpha either before or during exercise. In contrast, IL-6 increased throughout exercise in arterial plasma, and a net IL-6 release from the contracting limb was observed after 120 min of exercise (P < 0.05).

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Published In

Am J Physiol Endocrinol Metab

DOI

ISSN

0193-1849

Publication Date

December 2002

Volume

283

Issue

6

Start / End Page

E1272 / E1278

Location

United States

Related Subject Headings

  • Tumor Necrosis Factor-alpha
  • Regional Blood Flow
  • Reference Values
  • RNA, Messenger
  • Physical Exertion
  • Muscle, Skeletal
  • Muscle Contraction
  • Male
  • Leg
  • Interleukin-6
 

Citation

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Steensberg, A., Keller, C., Starkie, R. L., Osada, T., Febbraio, M. A., & Pedersen, B. K. (2002). IL-6 and TNF-alpha expression in, and release from, contracting human skeletal muscle. Am J Physiol Endocrinol Metab, 283(6), E1272–E1278. https://doi.org/10.1152/ajpendo.00255.2002
Steensberg, Adam, Charlotte Keller, Rebecca L. Starkie, Takuya Osada, Mark A. Febbraio, and Bente Klarlund Pedersen. “IL-6 and TNF-alpha expression in, and release from, contracting human skeletal muscle.Am J Physiol Endocrinol Metab 283, no. 6 (December 2002): E1272–78. https://doi.org/10.1152/ajpendo.00255.2002.
Steensberg A, Keller C, Starkie RL, Osada T, Febbraio MA, Pedersen BK. IL-6 and TNF-alpha expression in, and release from, contracting human skeletal muscle. Am J Physiol Endocrinol Metab. 2002 Dec;283(6):E1272–8.
Steensberg, Adam, et al. “IL-6 and TNF-alpha expression in, and release from, contracting human skeletal muscle.Am J Physiol Endocrinol Metab, vol. 283, no. 6, Dec. 2002, pp. E1272–78. Pubmed, doi:10.1152/ajpendo.00255.2002.
Steensberg A, Keller C, Starkie RL, Osada T, Febbraio MA, Pedersen BK. IL-6 and TNF-alpha expression in, and release from, contracting human skeletal muscle. Am J Physiol Endocrinol Metab. 2002 Dec;283(6):E1272–E1278.

Published In

Am J Physiol Endocrinol Metab

DOI

ISSN

0193-1849

Publication Date

December 2002

Volume

283

Issue

6

Start / End Page

E1272 / E1278

Location

United States

Related Subject Headings

  • Tumor Necrosis Factor-alpha
  • Regional Blood Flow
  • Reference Values
  • RNA, Messenger
  • Physical Exertion
  • Muscle, Skeletal
  • Muscle Contraction
  • Male
  • Leg
  • Interleukin-6