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Human epidermal plasminogen activator. Characterization, localization, and modulation.

Publication ,  Journal Article
Morioka, S; Jensen, PJ; Lazarus, GS
Published in: Exp Cell Res
December 1985

Using biochemical and immunocytochemical approaches, we have investigated the plasminogen activator (PA) of primary human epidermal cell cultures. A rabbit antibody raised against human urinary PA (urokinase) inhibited greater than or equal to 96% of the PA activity in the keratinocyte cultures. Immunoblot and double immunodiffusion analyses of keratinocyte PA with anti-urokinase antibody confirmed that epidermal PA was of the urokinase type. Immunocytochemical investigation of human keratinocyte cultures with anti-urokinase antibody revealed two characteristic staining patterns for PA. First, cells at the advancing edge of subconfluent colonies were cytoplasmically stained in a granular pattern. Similar staining was observed at the migrating edges of confluent epidermal cell cultures that had been wounded by cutting with a blade. This induction of PA staining was independent of cell division. Secondly, differentiated epidermal cells located on the surface of colonies were stained either at the plasma membrane or homogeneously throughout the cell. The highly differentiated, spontaneously shed cells were usually very heavily stained by anti-urokinase antibody. These immunocytochemical experiments suggest that PA expression is highly regulated in human epidermal cells. Specifically, PA expression appears to be related to cellular differentiation and to cell movement in expanding or wounded keratinocyte colonies.

Duke Scholars

Published In

Exp Cell Res

DOI

ISSN

0014-4827

Publication Date

December 1985

Volume

161

Issue

2

Start / End Page

364 / 372

Location

United States

Related Subject Headings

  • Urokinase-Type Plasminogen Activator
  • Plasminogen Activators
  • Penis
  • Male
  • Immunoenzyme Techniques
  • Humans
  • Epidermis
  • Cross Reactions
  • Cells, Cultured
  • Biochemistry & Molecular Biology
 

Citation

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Morioka, S., Jensen, P. J., & Lazarus, G. S. (1985). Human epidermal plasminogen activator. Characterization, localization, and modulation. Exp Cell Res, 161(2), 364–372. https://doi.org/10.1016/0014-4827(85)90093-x
Morioka, S., P. J. Jensen, and G. S. Lazarus. “Human epidermal plasminogen activator. Characterization, localization, and modulation.Exp Cell Res 161, no. 2 (December 1985): 364–72. https://doi.org/10.1016/0014-4827(85)90093-x.
Morioka S, Jensen PJ, Lazarus GS. Human epidermal plasminogen activator. Characterization, localization, and modulation. Exp Cell Res. 1985 Dec;161(2):364–72.
Morioka, S., et al. “Human epidermal plasminogen activator. Characterization, localization, and modulation.Exp Cell Res, vol. 161, no. 2, Dec. 1985, pp. 364–72. Pubmed, doi:10.1016/0014-4827(85)90093-x.
Morioka S, Jensen PJ, Lazarus GS. Human epidermal plasminogen activator. Characterization, localization, and modulation. Exp Cell Res. 1985 Dec;161(2):364–372.
Journal cover image

Published In

Exp Cell Res

DOI

ISSN

0014-4827

Publication Date

December 1985

Volume

161

Issue

2

Start / End Page

364 / 372

Location

United States

Related Subject Headings

  • Urokinase-Type Plasminogen Activator
  • Plasminogen Activators
  • Penis
  • Male
  • Immunoenzyme Techniques
  • Humans
  • Epidermis
  • Cross Reactions
  • Cells, Cultured
  • Biochemistry & Molecular Biology