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Hyperexpression of Foxp3 and IDO during acute rejection of islet allografts.

Publication ,  Journal Article
Yang, H; Ding, R; Sharma, VK; Hilaire, FS; Lagman, M; Li, B; Thomas, DA; Luo, X; Song, P; Stauffer, C; August, P; Suthanthiran, M
Published in: Transplantation
June 27, 2007

BACKGROUND: We investigated the hypothesis that Foxp3+ cells are an integral component of antiallograft immunity but are dominated by pathogenic effectors. METHODS: Wild-type H-2b C57BL/6 (B6) mice or B6 mice with a targeted disruption of c-Rel gene (c-Rel-/-) were used as recipients of islet grafts from allogeneic DBA/2 (H-2d) mice or syngeneic B6 mice. We developed kinetic quantitative polymerase chain reaction assays and measured intragraft expression of mRNA for Foxp3, IDO, cytolytic molecules, proinflammatory cytokines, and chemokines/receptors. RESULTS: Intraislet levels of mRNA for Foxp3, IDO, CD3, CD25, tumor necrosis factor-alpha, RANTES, IP-10, and CXCR3 were highest in DBA/2 islet allografts from WT B6 recipients compared to DBA/2 islet allografts from c-Rel-/- B6 recipients or syngeneic B6 islet grafts from WT B6 mice. The ratio of granzyme B or IFN-gamma to Foxp3 was higher with the DBA/2 islet allografts from the WT B6 recipients compared to DBA/2 islet allografts from c-Rel-/- B6 recipients or B6 islet grafts from WT B6 recipients. CONCLUSIONS: Foxp3+ cells are an integral component of acute rejection of allografts but may be dominated by pathogenic effectors.

Duke Scholars

Published In

Transplantation

DOI

ISSN

0041-1337

Publication Date

June 27, 2007

Volume

83

Issue

12

Start / End Page

1643 / 1647

Location

United States

Related Subject Headings

  • Surgery
  • RNA, Messenger
  • Proto-Oncogene Proteins c-rel
  • Models, Animal
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Mice
  • Islets of Langerhans Transplantation
  • Indoleamine-Pyrrole 2,3,-Dioxygenase
  • Granzymes
 

Citation

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Yang, H., Ding, R., Sharma, V. K., Hilaire, F. S., Lagman, M., Li, B., … Suthanthiran, M. (2007). Hyperexpression of Foxp3 and IDO during acute rejection of islet allografts. Transplantation, 83(12), 1643–1647. https://doi.org/10.1097/01.tp.0000263991.74052.46
Yang, Hua, Ruchuang Ding, Vijay K. Sharma, Fludd Saint Hilaire, Milagros Lagman, Baogui Li, Dolca A. Thomas, et al. “Hyperexpression of Foxp3 and IDO during acute rejection of islet allografts.Transplantation 83, no. 12 (June 27, 2007): 1643–47. https://doi.org/10.1097/01.tp.0000263991.74052.46.
Yang H, Ding R, Sharma VK, Hilaire FS, Lagman M, Li B, et al. Hyperexpression of Foxp3 and IDO during acute rejection of islet allografts. Transplantation. 2007 Jun 27;83(12):1643–7.
Yang, Hua, et al. “Hyperexpression of Foxp3 and IDO during acute rejection of islet allografts.Transplantation, vol. 83, no. 12, June 2007, pp. 1643–47. Pubmed, doi:10.1097/01.tp.0000263991.74052.46.
Yang H, Ding R, Sharma VK, Hilaire FS, Lagman M, Li B, Thomas DA, Luo X, Song P, Stauffer C, August P, Suthanthiran M. Hyperexpression of Foxp3 and IDO during acute rejection of islet allografts. Transplantation. 2007 Jun 27;83(12):1643–1647.

Published In

Transplantation

DOI

ISSN

0041-1337

Publication Date

June 27, 2007

Volume

83

Issue

12

Start / End Page

1643 / 1647

Location

United States

Related Subject Headings

  • Surgery
  • RNA, Messenger
  • Proto-Oncogene Proteins c-rel
  • Models, Animal
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Mice
  • Islets of Langerhans Transplantation
  • Indoleamine-Pyrrole 2,3,-Dioxygenase
  • Granzymes