Both NaCl and H₂O₂ Long-Term Stresses Affect Basal Cytosolic Ca²⁺ Levels but Only NaCl Alters Cytosolic Ca²⁺ Signatures in Arabidopsis.

Salinity is one of the formidable environmental factors that affect plant growth and development and constrain agricultural productivity. Experimentally imposed short-term NaCl treatment triggers a transient increase in cytosolic free Ca²⁺ concentration ([Ca²⁺]_i) via Ca²⁺ influx across the plasma membrane. Salinity stress, as well as other stresses, induces the production of reactive oxygen species (ROS), such as H₂O₂. It is well established that short-term H₂O₂ treatment also triggers a transient increase in [Ca²⁺]_i. However, whether and how long-term NaCl and H₂O₂ treatments affect the basal levels of [Ca²⁺]_i as well as plant responses to additional NaCl and H₂O₂ stresses remain poorly understood. Using an aequorin-based Ca²⁺ imaging assay, we found that the long-term treatment of Arabidopsis seedlings with both moderate NaCl and H₂O₂ in the growth media reduced the basal [Ca²⁺]_i levels. Interestingly, we found that the long-term treatment with NaCl, but not H₂O_2, affected the responses of plants to additional NaCl stress, and remarkably the roots displayed enhanced responses while the leaves showed reduced responses. These findings suggest that plants adapt to the long-term NaCl stress, while H₂O₂ might be an integrator of many stresses.

Duke Scholars

Author Zhen-Ming Pei Biology