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The case for thyroid disruption in early life stage exposures to thiram in zebrafish (Danio rerio).

Publication ,  Journal Article
Chen, X; Fang, M; Chernick, M; Wang, F; Yang, J; Yu, Y; Zheng, N; Teraoka, H; Nanba, S; Hiraga, T; Hinton, DE; Dong, W
Published in: General and comparative endocrinology
January 2019

Thiram, a pesticide in the dithiocarbamate chemical family, is widely used to prevent fungal disease in seeds and crops. Its off-site movement to surface waters occurs and may place aquatic organisms at potential harm. Zebrafish embryos were used for investigation of acute (1 h) thiram exposure (0.001-10 µM) at various developmental stages. Survival decreased at 1 µM and 10 µM and hatching was delayed at 0.1 µM and 1 µM. Notochord curvatures were seen at 0.1 and 1 μM thiram when exposure was initiated at 2 and at 10 hpf. Similar notochord curvatures followed exposure to the known TPO inhibitor, methimazole (MMI). Changes were absent in embryos exposed at later stages, i.e., 12 hpf. In embryos exposed to 0.1 or 1 μM at 10 hpf, levels of the thyroid enzyme, Deiodinase 3, increased by 12 hpf. Thyroid peroxide (TPO), important in T4 synthesis, decreased by 48 hpf in embryos exposed to 1 µM at 10 hpf. Thiram toxicity was stage-dependent and early life stage exposure may be responsible for adverse effects seen later. These effects may be due to impacts on the thyroid via regulation of specific thyroid genes including TPO and Deiodinase 3.

Duke Scholars

Published In

General and comparative endocrinology

DOI

EISSN

1095-6840

ISSN

0016-6480

Publication Date

January 2019

Volume

271

Start / End Page

73 / 81

Related Subject Headings

  • Zebrafish Proteins
  • Zebrafish
  • Thyroid Gland
  • Thiram
  • RNA, Messenger
  • Larva
  • Iodide Peroxidase
  • Gene Expression Regulation, Developmental
  • Endocrinology & Metabolism
  • Embryo, Nonmammalian
 

Citation

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Chen, X., Fang, M., Chernick, M., Wang, F., Yang, J., Yu, Y., … Dong, W. (2019). The case for thyroid disruption in early life stage exposures to thiram in zebrafish (Danio rerio). General and Comparative Endocrinology, 271, 73–81. https://doi.org/10.1016/j.ygcen.2018.11.003
Chen, Xing, Mingliang Fang, Melissa Chernick, Feng Wang, Jingfeng Yang, Yongli Yu, Na Zheng, et al. “The case for thyroid disruption in early life stage exposures to thiram in zebrafish (Danio rerio).General and Comparative Endocrinology 271 (January 2019): 73–81. https://doi.org/10.1016/j.ygcen.2018.11.003.
Chen X, Fang M, Chernick M, Wang F, Yang J, Yu Y, et al. The case for thyroid disruption in early life stage exposures to thiram in zebrafish (Danio rerio). General and comparative endocrinology. 2019 Jan;271:73–81.
Chen, Xing, et al. “The case for thyroid disruption in early life stage exposures to thiram in zebrafish (Danio rerio).General and Comparative Endocrinology, vol. 271, Jan. 2019, pp. 73–81. Epmc, doi:10.1016/j.ygcen.2018.11.003.
Chen X, Fang M, Chernick M, Wang F, Yang J, Yu Y, Zheng N, Teraoka H, Nanba S, Hiraga T, Hinton DE, Dong W. The case for thyroid disruption in early life stage exposures to thiram in zebrafish (Danio rerio). General and comparative endocrinology. 2019 Jan;271:73–81.
Journal cover image

Published In

General and comparative endocrinology

DOI

EISSN

1095-6840

ISSN

0016-6480

Publication Date

January 2019

Volume

271

Start / End Page

73 / 81

Related Subject Headings

  • Zebrafish Proteins
  • Zebrafish
  • Thyroid Gland
  • Thiram
  • RNA, Messenger
  • Larva
  • Iodide Peroxidase
  • Gene Expression Regulation, Developmental
  • Endocrinology & Metabolism
  • Embryo, Nonmammalian