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Optogenetic manipulation of neural circuits in awake marmosets

Publication ,  Journal Article
MacDougall, M; Nummela, SU; Coop, S; Disney, A; Mitchell, JF; Miller, CT
Published in: Journal of Neurophysiology
September 1, 2016

Optogenetics has revolutionized the study of functional neuronal circuitry (Boyden ES, Zhang F, Bamberg E, Nagel G, Deisseroth K. Nat Neurosci 8: 1263–1268, 2005; Deisseroth K. Nat Methods 8: 26–29, 2011). Although these techniques have been most successfully implemented in rodent models, they have the potential to be similarly impactful in studies of nonhuman primate brains. Common marmosets ( Callithrix jacchus) have recently emerged as a candidate primate model for gene editing, providing a potentially powerful model for studies of neural circuitry and disease in primates. The application of viral transduction methods in marmosets for identifying and manipulating neuronal circuitry is a crucial step in developing this species for neuroscience research. In the present study we developed a novel, chronic method to successfully induce rapid photostimulation in individual cortical neurons transduced by adeno-associated virus to express channelrhodopsin (ChR2) in awake marmosets. We found that large proportions of neurons could be effectively photoactivated following viral transduction and that this procedure could be repeated for several months. These data suggest that techniques for viral transduction and optical manipulation of neuronal populations are suitable for marmosets and can be combined with existing behavioral preparations in the species to elucidate the functional neural circuitry underlying perceptual and cognitive processes.

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Published In

Journal of Neurophysiology

DOI

EISSN

1522-1598

ISSN

0022-3077

Publication Date

September 1, 2016

Volume

116

Issue

3

Start / End Page

1286 / 1294

Publisher

American Physiological Society

Related Subject Headings

  • Neurology & Neurosurgery
  • 17 Psychology and Cognitive Sciences
  • 11 Medical and Health Sciences
 

Citation

APA
Chicago
ICMJE
MLA
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MacDougall, M., Nummela, S. U., Coop, S., Disney, A., Mitchell, J. F., & Miller, C. T. (2016). Optogenetic manipulation of neural circuits in awake marmosets. Journal of Neurophysiology, 116(3), 1286–1294. https://doi.org/10.1152/jn.00197.2016
MacDougall, Matthew, Samuel U. Nummela, Shanna Coop, Anita Disney, Jude F. Mitchell, and Cory T. Miller. “Optogenetic manipulation of neural circuits in awake marmosets.” Journal of Neurophysiology 116, no. 3 (September 1, 2016): 1286–94. https://doi.org/10.1152/jn.00197.2016.
MacDougall M, Nummela SU, Coop S, Disney A, Mitchell JF, Miller CT. Optogenetic manipulation of neural circuits in awake marmosets. Journal of Neurophysiology. 2016 Sep 1;116(3):1286–94.
MacDougall, Matthew, et al. “Optogenetic manipulation of neural circuits in awake marmosets.” Journal of Neurophysiology, vol. 116, no. 3, American Physiological Society, Sept. 2016, pp. 1286–94. Crossref, doi:10.1152/jn.00197.2016.
MacDougall M, Nummela SU, Coop S, Disney A, Mitchell JF, Miller CT. Optogenetic manipulation of neural circuits in awake marmosets. Journal of Neurophysiology. American Physiological Society; 2016 Sep 1;116(3):1286–1294.

Published In

Journal of Neurophysiology

DOI

EISSN

1522-1598

ISSN

0022-3077

Publication Date

September 1, 2016

Volume

116

Issue

3

Start / End Page

1286 / 1294

Publisher

American Physiological Society

Related Subject Headings

  • Neurology & Neurosurgery
  • 17 Psychology and Cognitive Sciences
  • 11 Medical and Health Sciences