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High-Throughput Video Processing of Heart Rate Responses in Multiple Wild-type Embryonic Zebrafish per Imaging Field.

Publication ,  Journal Article
Martin, WK; Tennant, AH; Conolly, RB; Prince, K; Stevens, JS; DeMarini, DM; Martin, BL; Thompson, LC; Gilmour, MI; Cascio, WE; Hays, MD ...
Published in: Scientific reports
January 2019

Heart rate assays in wild-type zebrafish embryos have been limited to analysis of one embryo per video/imaging field. Here we present for the first time a platform for high-throughput derivation of heart rate from multiple zebrafish (Danio rerio) embryos per imaging field, which is capable of quickly processing thousands of videos and ideal for multi-well platforms with multiple fish/well. This approach relies on use of 2-day post fertilization wild-type embryos, and uses only bright-field imaging, circumventing requirement for anesthesia or restraint, costly software/hardware, or fluorescently-labeled animals. Our original scripts (1) locate the heart and record pixel intensity fluctuations generated by each cardiac cycle using a robust image processing routine, and (2) process intensity data to derive heart rate. To demonstrate assay utility, we exposed embryos to the drugs epinephrine and clonidine, which increased or decreased heart rate, respectively. Exposure to organic extracts of air pollution-derived particulate matter, including diesel or biodiesel exhausts, or wood smoke, all complex environmental mixtures, decreased heart rate to varying degrees. Comparison against an established lower-throughput method indicated robust assay fidelity. As all code and executable files are publicly available, this approach may expedite cardiotoxicity screening of compounds as diverse as small molecule drugs and complex chemical mixtures.

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Published In

Scientific reports

DOI

EISSN

2045-2322

ISSN

2045-2322

Publication Date

January 2019

Volume

9

Issue

1

Start / End Page

145

Related Subject Headings

  • Zebrafish
  • Particulate Matter
  • Image Processing, Computer-Assisted
  • High-Throughput Screening Assays
  • Heart Rate
  • Embryo, Nonmammalian
  • Drug Evaluation, Preclinical
  • Cardiotoxicity
  • Animals
 

Citation

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Martin, W. K., Tennant, A. H., Conolly, R. B., Prince, K., Stevens, J. S., DeMarini, D. M., … Farraj, A. K. (2019). High-Throughput Video Processing of Heart Rate Responses in Multiple Wild-type Embryonic Zebrafish per Imaging Field. Scientific Reports, 9(1), 145. https://doi.org/10.1038/s41598-018-35949-5
Martin, W Kyle, Alan H. Tennant, Rory B. Conolly, Katya Prince, Joey S. Stevens, David M. DeMarini, Brandi L. Martin, et al. “High-Throughput Video Processing of Heart Rate Responses in Multiple Wild-type Embryonic Zebrafish per Imaging Field.Scientific Reports 9, no. 1 (January 2019): 145. https://doi.org/10.1038/s41598-018-35949-5.
Martin WK, Tennant AH, Conolly RB, Prince K, Stevens JS, DeMarini DM, et al. High-Throughput Video Processing of Heart Rate Responses in Multiple Wild-type Embryonic Zebrafish per Imaging Field. Scientific reports. 2019 Jan;9(1):145.
Martin, W. Kyle, et al. “High-Throughput Video Processing of Heart Rate Responses in Multiple Wild-type Embryonic Zebrafish per Imaging Field.Scientific Reports, vol. 9, no. 1, Jan. 2019, p. 145. Epmc, doi:10.1038/s41598-018-35949-5.
Martin WK, Tennant AH, Conolly RB, Prince K, Stevens JS, DeMarini DM, Martin BL, Thompson LC, Gilmour MI, Cascio WE, Hays MD, Hazari MS, Padilla S, Farraj AK. High-Throughput Video Processing of Heart Rate Responses in Multiple Wild-type Embryonic Zebrafish per Imaging Field. Scientific reports. 2019 Jan;9(1):145.

Published In

Scientific reports

DOI

EISSN

2045-2322

ISSN

2045-2322

Publication Date

January 2019

Volume

9

Issue

1

Start / End Page

145

Related Subject Headings

  • Zebrafish
  • Particulate Matter
  • Image Processing, Computer-Assisted
  • High-Throughput Screening Assays
  • Heart Rate
  • Embryo, Nonmammalian
  • Drug Evaluation, Preclinical
  • Cardiotoxicity
  • Animals