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Post‐translational modification directs nuclear and hyphal tip localization of andida albicans m‐binding protein lr1

Publication ,  Journal Article
Ariyachet, C; Beißel, C; Li, X; Lorrey, S; Mackenzie, O; Martin, PM; O'Brien, K; Pholcharee, T; Sim, S; Krebber, H; McBride, AE
Published in: Molecular Microbiology
May 2017

The morphological transition of the opportunistic fungal pathogen from budding to hyphal growth has been implicated in its ability to cause disease in animal models. Absence of R‐ike NA‐binding protein Slr1 slows hyphal formation and decreases virulence in a systemic candidiasis model, suggesting a role for post‐transcriptional regulation in these processes. SR (serine–arginine)‐rich proteins influence multiple steps in mRNA metabolism and their localization and function are frequently controlled by modification. We now demonstrate that Slr1 binds to polyadenylated RNA and that its intracellular localization is modulated by phosphorylation and methylation. Wildtype Slr1‐GFP is predominantly nuclear, but also co‐fractionates with translating ribosomes. The non‐phosphorylatable slr1‐6SA‐GFP protein, in which six serines in SR/RS clusters are substituted with alanines, primarily localizes to the cytoplasm in budding cells. Intriguingly, hyphal cells display a slr1‐6SA‐GFP focus at the tip near the Spitzenkörper, a vesicular structure involved in molecular trafficking to the tip. The presence of slr1‐6SA‐GFP hyphal tip foci is reduced in the absence of the mRNA‐transport protein She3, suggesting that unphosphorylated Slr1 associates with mRNA–protein complexes transported to the tip. The impact of deletion on hyphal formation and function thus may be partially due to a role in hyphal mRNA transport.

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Published In

Molecular Microbiology

DOI

EISSN

1365-2958

ISSN

0950-382X

Publication Date

May 2017

Volume

104

Issue

3

Start / End Page

499 / 519

Publisher

Wiley

Related Subject Headings

  • Microbiology
  • 11 Medical and Health Sciences
  • 07 Agricultural and Veterinary Sciences
  • 06 Biological Sciences
 

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Ariyachet, C., Beißel, C., Li, X., Lorrey, S., Mackenzie, O., Martin, P. M., … McBride, A. E. (2017). Post‐translational modification directs nuclear and hyphal tip localization of Candida albicans mRNA‐binding protein Slr1. Molecular Microbiology, 104(3), 499–519. https://doi.org/10.1111/mmi.13643
Ariyachet, Chaiyaboot, Christian Beißel, Xiang Li, Selena Lorrey, Olivia Mackenzie, Patrick M. Martin, Katharine O’Brien, et al. “Post‐translational modification directs nuclear and hyphal tip localization of Candida albicans mRNA‐binding protein Slr1.” Molecular Microbiology 104, no. 3 (May 2017): 499–519. https://doi.org/10.1111/mmi.13643.
Ariyachet C, Beißel C, Li X, Lorrey S, Mackenzie O, Martin PM, et al. Post‐translational modification directs nuclear and hyphal tip localization of Candida albicans mRNA‐binding protein Slr1. Molecular Microbiology. 2017 May;104(3):499–519.
Ariyachet, Chaiyaboot, et al. “Post‐translational modification directs nuclear and hyphal tip localization of Candida albicans mRNA‐binding protein Slr1.” Molecular Microbiology, vol. 104, no. 3, Wiley, May 2017, pp. 499–519. Crossref, doi:10.1111/mmi.13643.
Ariyachet C, Beißel C, Li X, Lorrey S, Mackenzie O, Martin PM, O’Brien K, Pholcharee T, Sim S, Krebber H, McBride AE. Post‐translational modification directs nuclear and hyphal tip localization of Candida albicans mRNA‐binding protein Slr1. Molecular Microbiology. Wiley; 2017 May;104(3):499–519.
Journal cover image

Published In

Molecular Microbiology

DOI

EISSN

1365-2958

ISSN

0950-382X

Publication Date

May 2017

Volume

104

Issue

3

Start / End Page

499 / 519

Publisher

Wiley

Related Subject Headings

  • Microbiology
  • 11 Medical and Health Sciences
  • 07 Agricultural and Veterinary Sciences
  • 06 Biological Sciences