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Partial liquid ventilation with perflubron attenuates in vivo oxidative damage to proteins and lipids.

Publication ,  Journal Article
Rotta, AT; Gunnarsson, B; Hernan, LJ; Fuhrman, BP; Steinhorn, DM
Published in: Crit Care Med
January 2000

OBJECTIVE: To determine the impact of partial liquid ventilation on the degree of pulmonary damage by reactive oxygen species in a model of acute lung injury caused by systemic endotoxemia. DESIGN: A prospective, controlled, in vivo, animal laboratory study. SETTING: Animal research facility of a health sciences university. SUBJECTS: Forty New Zealand White rabbits. INTERVENTIONS: Mature rabbits were anesthetized and instrumented with a tracheostomy and vascular catheters. Animals were assigned to receive either partial liquid ventilation (n = 16) with perflubron (18 mL/kg via endotracheal tube) or conventional mechanical ventilation (n = 16). Both groups were ventilated using similar strategies, with an Fio2 of 1.0 and tidal volume as required to obtain a normal Paco2. Animals were then given 0.9 mg/kg Escherichia coli endotoxin intravenously over 30 mins. Eight uninjured instrumented and mechanically ventilated animals served as controls. Partial liquid ventilation or conventional ventilation was continued for 4 hrs before the animals were killed. Lung homogenates were analyzed for malondialdehyde (MDA) and 4-hydroxy-2(E)-nonenal (4-HNE) concentrations using a colorimetric assay. To assess protein oxidative damage, carbonyl groups in protein side chains were derivatized with 2,4-dinitrophenylhydrazine followed by Western blotting with a dinitrophenylated-specific primary antibody. MEASUREMENTS AND MAIN RESULTS: MDA (713.42+/-662 vs. 1601.4+/-1156 nmol/g protein; p = .023) and MDA plus 4-HNE (1480.24+/-788 vs. 2675.2+/-1628 nmol/g protein; p = .038) concentrations were lower in animals treated with partial liquid ventilation compared with conventionally ventilated animals, respectively. Animals treated with partial liquid ventilation exhibited attenuation of dinitrophenylated-derivatized protein bands by Western blotting, indicating a reduction in protein oxidative damage. The presence of perfluorocarbon did not interfere with the MDA assay when assessed by independent analysis in vitro. Perflubron did not serve as a sink for peroxyl radicals produced in the aqueous phase during separate in vitro oxidation experiments. CONCLUSIONS: Partial liquid ventilation attenuates oxidative damage to lipids and proteins during experimental acute lung injury. This finding is not caused by binding of lipid peroxidation products to perflubron or by the peroxyl radical scavenging properties of perflubron.

Duke Scholars

Published In

Crit Care Med

DOI

ISSN

0090-3493

Publication Date

January 2000

Volume

28

Issue

1

Start / End Page

202 / 208

Location

United States

Related Subject Headings

  • Respiratory Function Tests
  • Respiratory Distress Syndrome
  • Respiration, Artificial
  • Rabbits
  • Proteins
  • Prospective Studies
  • Malondialdehyde
  • Lipid Peroxidation
  • Hydrocarbons, Brominated
  • Fluorocarbons
 

Citation

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ICMJE
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Rotta, A. T., Gunnarsson, B., Hernan, L. J., Fuhrman, B. P., & Steinhorn, D. M. (2000). Partial liquid ventilation with perflubron attenuates in vivo oxidative damage to proteins and lipids. Crit Care Med, 28(1), 202–208. https://doi.org/10.1097/00003246-200001000-00033
Rotta, A. T., B. Gunnarsson, L. J. Hernan, B. P. Fuhrman, and D. M. Steinhorn. “Partial liquid ventilation with perflubron attenuates in vivo oxidative damage to proteins and lipids.Crit Care Med 28, no. 1 (January 2000): 202–8. https://doi.org/10.1097/00003246-200001000-00033.
Rotta AT, Gunnarsson B, Hernan LJ, Fuhrman BP, Steinhorn DM. Partial liquid ventilation with perflubron attenuates in vivo oxidative damage to proteins and lipids. Crit Care Med. 2000 Jan;28(1):202–8.
Rotta, A. T., et al. “Partial liquid ventilation with perflubron attenuates in vivo oxidative damage to proteins and lipids.Crit Care Med, vol. 28, no. 1, Jan. 2000, pp. 202–08. Pubmed, doi:10.1097/00003246-200001000-00033.
Rotta AT, Gunnarsson B, Hernan LJ, Fuhrman BP, Steinhorn DM. Partial liquid ventilation with perflubron attenuates in vivo oxidative damage to proteins and lipids. Crit Care Med. 2000 Jan;28(1):202–208.

Published In

Crit Care Med

DOI

ISSN

0090-3493

Publication Date

January 2000

Volume

28

Issue

1

Start / End Page

202 / 208

Location

United States

Related Subject Headings

  • Respiratory Function Tests
  • Respiratory Distress Syndrome
  • Respiration, Artificial
  • Rabbits
  • Proteins
  • Prospective Studies
  • Malondialdehyde
  • Lipid Peroxidation
  • Hydrocarbons, Brominated
  • Fluorocarbons