Diversification of the TCR β Locus Vβ Repertoire by CTCF.
Publication
, Journal Article
Chen, S; Krangel, MS
Published in: Immunohorizons
December 2018
The architectural protein CTCF regulates the conformation and recombination of antigen receptor loci. To study the importance of CTCF in Tcrb locus repertoire formation, we created a conditional knockout mouse line that deletes Ctcf early during thymocyte development. We observed an incomplete block in thymocyte development at the double-negative to double-positive transition, resulting in greatly lowered thymic cellularity. The Tcrb repertoire was altered with a decrease in recombination of Vβ gene segments in close proximity to a CTCF binding element (CBE), resulting in an overall repertoire that was skewed in favor of Vβ gene segments with no nearby CBE. Therefore we show that CTCF functions to diversify the Tcrb repertoire.
Duke Scholars
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Published In
Immunohorizons
DOI
ISSN
2573-7732
Publication Date
December 2018
Volume
2
Issue
11
Start / End Page
377 / 383
Location
United States
Citation
APA
Chicago
ICMJE
MLA
NLM
Chen, S., & Krangel, M. S. (2018). Diversification of the TCR β Locus Vβ Repertoire by CTCF. Immunohorizons, 2(11), 377–383. https://doi.org/10.4049/immunohorizons.1800072
Chen, Shiwei, and Michael S. Krangel. “Diversification of the TCR β Locus Vβ Repertoire by CTCF.” Immunohorizons 2, no. 11 (December 2018): 377–83. https://doi.org/10.4049/immunohorizons.1800072.
Chen S, Krangel MS. Diversification of the TCR β Locus Vβ Repertoire by CTCF. Immunohorizons. 2018 Dec;2(11):377–83.
Chen, Shiwei, and Michael S. Krangel. “Diversification of the TCR β Locus Vβ Repertoire by CTCF.” Immunohorizons, vol. 2, no. 11, Dec. 2018, pp. 377–83. Pubmed, doi:10.4049/immunohorizons.1800072.
Chen S, Krangel MS. Diversification of the TCR β Locus Vβ Repertoire by CTCF. Immunohorizons. 2018 Dec;2(11):377–383.
Published In
Immunohorizons
DOI
ISSN
2573-7732
Publication Date
December 2018
Volume
2
Issue
11
Start / End Page
377 / 383
Location
United States