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Fungal-Bacterial Networks in the Populus Rhizobiome Are Impacted by Soil Properties and Host Genotype.

Publication ,  Journal Article
Bonito, G; Benucci, GMN; Hameed, K; Weighill, D; Jones, P; Chen, K-H; Jacobson, D; Schadt, C; Vilgalys, R
Published in: Frontiers in microbiology
January 2019

Plant root-associated microbial symbionts comprise the plant rhizobiome. These microbes function in provisioning nutrients and water to their hosts, impacting plant health and disease. The plant microbiome is shaped by plant species, plant genotype, soil and environmental conditions, but the contributions of these variables are hard to disentangle from each other in natural systems. We used bioassay common garden experiments to decouple plant genotype and soil property impacts on fungal and bacterial community structure in the Populus rhizobiome. High throughput amplification and sequencing of 16S, ITS, 28S and 18S rDNA was accomplished through 454 pyrosequencing. Co-association patterns of fungal and bacterial taxa were assessed with 16S and ITS datasets. Community bipartite fungal-bacterial networks and PERMANOVA results attribute significant difference in fungal or bacterial communities to soil origin, soil chemical properties and plant genotype. Indicator species analysis identified a common set of root bacteria as well as endophytic and ectomycorrhizal fungi associated with Populus in different soils. However, no single taxon, or consortium of microbes, was indicative of a particular Populus genotype. Fungal-bacterial networks were over-represented in arbuscular mycorrhizal, endophytic, and ectomycorrhizal fungi, as well as bacteria belonging to the orders Rhizobiales, Chitinophagales, Cytophagales, and Burkholderiales. These results demonstrate the importance of soil and plant genotype on fungal-bacterial networks in the belowground plant microbiome.

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Published In

Frontiers in microbiology

DOI

EISSN

1664-302X

ISSN

1664-302X

Publication Date

January 2019

Volume

10

Start / End Page

481

Related Subject Headings

  • 3207 Medical microbiology
  • 3107 Microbiology
  • 0605 Microbiology
  • 0503 Soil Sciences
  • 0502 Environmental Science and Management
 

Citation

APA
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ICMJE
MLA
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Bonito, G., Benucci, G. M. N., Hameed, K., Weighill, D., Jones, P., Chen, K.-H., … Vilgalys, R. (2019). Fungal-Bacterial Networks in the Populus Rhizobiome Are Impacted by Soil Properties and Host Genotype. Frontiers in Microbiology, 10, 481. https://doi.org/10.3389/fmicb.2019.00481
Bonito, Gregory, Gian Maria Niccolò Benucci, Khalid Hameed, Deborah Weighill, Piet Jones, Ko-Hsuan Chen, Daniel Jacobson, Christopher Schadt, and Rytas Vilgalys. “Fungal-Bacterial Networks in the Populus Rhizobiome Are Impacted by Soil Properties and Host Genotype.Frontiers in Microbiology 10 (January 2019): 481. https://doi.org/10.3389/fmicb.2019.00481.
Bonito G, Benucci GMN, Hameed K, Weighill D, Jones P, Chen K-H, et al. Fungal-Bacterial Networks in the Populus Rhizobiome Are Impacted by Soil Properties and Host Genotype. Frontiers in microbiology. 2019 Jan;10:481.
Bonito, Gregory, et al. “Fungal-Bacterial Networks in the Populus Rhizobiome Are Impacted by Soil Properties and Host Genotype.Frontiers in Microbiology, vol. 10, Jan. 2019, p. 481. Epmc, doi:10.3389/fmicb.2019.00481.
Bonito G, Benucci GMN, Hameed K, Weighill D, Jones P, Chen K-H, Jacobson D, Schadt C, Vilgalys R. Fungal-Bacterial Networks in the Populus Rhizobiome Are Impacted by Soil Properties and Host Genotype. Frontiers in microbiology. 2019 Jan;10:481.

Published In

Frontiers in microbiology

DOI

EISSN

1664-302X

ISSN

1664-302X

Publication Date

January 2019

Volume

10

Start / End Page

481

Related Subject Headings

  • 3207 Medical microbiology
  • 3107 Microbiology
  • 0605 Microbiology
  • 0503 Soil Sciences
  • 0502 Environmental Science and Management