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Simultaneous analysis of allopurinol and oxypurinol using a validated liquid chromatography–tandem mass spectrometry method in human plasma

Publication ,  Journal Article
Rathod, DM; Patel, KR; Mistri, HN; Jangid, AG; Shrivastav, PS; Sanyal, M
Published in: Journal of Pharmaceutical Analysis
February 1, 2017

The present study describes a simple, reliable and reproducible liquid chromatography–tandem mass spectrometry method (LC–MS/MS) for the simultaneous determination of allopurinol and its active metabolite, oxypurinol in human plasma for a pharmacokinetic/bioequivalence study. After protein precipitation (PPT) of 100 µL plasma sample with 1.0% formic acid in acetonitrile, the recovery of the analytes and allopurinol-d2 as an internal standard ranged from 85.36% to 91.20%. The analytes were separated on Hypersil Gold (150 mm×4.6 mm, 5 µm) column using 0.1% formic acid-acetonitrile (98:2, v/v) as the mobile phase. Quantification was done using electrospray ionization in the positive mode. The calibration concentration range was established from 60.0 to 6000 ng/mL for allopurinol and 80.0–8000 ng/mL for oxypurinol. Matrix effect in human plasma, expressed as IS-normalized matrix factors ranged from 1.003 to 1.030 for both the analytes. The developed method was found suitable for a clinical study with 300 mg allopurinol tablet formulation in healthy subjects.

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Published In

Journal of Pharmaceutical Analysis

DOI

ISSN

2095-1779

Publication Date

February 1, 2017

Volume

7

Issue

1

Start / End Page

56 / 62
 

Citation

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Rathod, D. M., Patel, K. R., Mistri, H. N., Jangid, A. G., Shrivastav, P. S., & Sanyal, M. (2017). Simultaneous analysis of allopurinol and oxypurinol using a validated liquid chromatography–tandem mass spectrometry method in human plasma. Journal of Pharmaceutical Analysis, 7(1), 56–62. https://doi.org/10.1016/j.jpha.2016.05.005
Rathod, D. M., K. R. Patel, H. N. Mistri, A. G. Jangid, P. S. Shrivastav, and M. Sanyal. “Simultaneous analysis of allopurinol and oxypurinol using a validated liquid chromatography–tandem mass spectrometry method in human plasma.” Journal of Pharmaceutical Analysis 7, no. 1 (February 1, 2017): 56–62. https://doi.org/10.1016/j.jpha.2016.05.005.
Rathod DM, Patel KR, Mistri HN, Jangid AG, Shrivastav PS, Sanyal M. Simultaneous analysis of allopurinol and oxypurinol using a validated liquid chromatography–tandem mass spectrometry method in human plasma. Journal of Pharmaceutical Analysis. 2017 Feb 1;7(1):56–62.
Rathod, D. M., et al. “Simultaneous analysis of allopurinol and oxypurinol using a validated liquid chromatography–tandem mass spectrometry method in human plasma.” Journal of Pharmaceutical Analysis, vol. 7, no. 1, Feb. 2017, pp. 56–62. Scopus, doi:10.1016/j.jpha.2016.05.005.
Rathod DM, Patel KR, Mistri HN, Jangid AG, Shrivastav PS, Sanyal M. Simultaneous analysis of allopurinol and oxypurinol using a validated liquid chromatography–tandem mass spectrometry method in human plasma. Journal of Pharmaceutical Analysis. 2017 Feb 1;7(1):56–62.
Journal cover image

Published In

Journal of Pharmaceutical Analysis

DOI

ISSN

2095-1779

Publication Date

February 1, 2017

Volume

7

Issue

1

Start / End Page

56 / 62