Skip to main content

Homologous Recombination and Translesion DNA Synthesis Play Critical Roles on Tolerating DNA Damage Caused by Trace Levels of Hexavalent Chromium.

Publication ,  Journal Article
Tian, X; Patel, K; Ridpath, JR; Chen, Y; Zhou, Y-H; Neo, D; Clement, J; Takata, M; Takeda, S; Sale, J; Wright, FA; Swenberg, JA; Nakamura, J
Published in: PLoS One
2016

Contamination of potentially carcinogenic hexavalent chromium (Cr(VI)) in the drinking water is a major public health concern worldwide. However, little information is available regarding the biological effects of a nanomoler amount of Cr(VI). Here, we investigated the genotoxic effects of Cr(VI) at nanomoler levels and their repair pathways. We found that DNA damage response analyzed based on differential toxicity of isogenic cells deficient in various DNA repair proteins is observed after a three-day incubation with K2CrO4 in REV1-deficient DT40 cells at 19.2 μg/L or higher as well as in TK6 cells deficient in polymerase delta subunit 3 (POLD3) at 9.8 μg/L or higher. The genotoxicity of Cr(VI) decreased ~3000 times when the incubation time was reduced from three days to ten minutes. TK mutation rate also significantly decreased from 6 day to 1 day exposure to Cr(VI). The DNA damage response analysis suggest that DNA repair pathways, including the homologous recombination and REV1- and POLD3-mediated error-prone translesion synthesis pathways, are critical for the cells to tolerate to DNA damage caused by trace amount of Cr(VI).

Duke Scholars

Published In

PLoS One

DOI

EISSN

1932-6203

Publication Date

2016

Volume

11

Issue

12

Start / End Page

e0167503

Location

United States

Related Subject Headings

  • Time Factors
  • Mutation
  • Humans
  • Homologous Recombination
  • General Science & Technology
  • Dose-Response Relationship, Drug
  • DNA Replication
  • DNA Damage
  • Chromium
  • Chickens
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Tian, X., Patel, K., Ridpath, J. R., Chen, Y., Zhou, Y.-H., Neo, D., … Nakamura, J. (2016). Homologous Recombination and Translesion DNA Synthesis Play Critical Roles on Tolerating DNA Damage Caused by Trace Levels of Hexavalent Chromium. PLoS One, 11(12), e0167503. https://doi.org/10.1371/journal.pone.0167503
Tian, Xu, Keyur Patel, John R. Ridpath, Youjun Chen, Yi-Hui Zhou, Dayna Neo, Jean Clement, et al. “Homologous Recombination and Translesion DNA Synthesis Play Critical Roles on Tolerating DNA Damage Caused by Trace Levels of Hexavalent Chromium.PLoS One 11, no. 12 (2016): e0167503. https://doi.org/10.1371/journal.pone.0167503.
Tian, Xu, et al. “Homologous Recombination and Translesion DNA Synthesis Play Critical Roles on Tolerating DNA Damage Caused by Trace Levels of Hexavalent Chromium.PLoS One, vol. 11, no. 12, 2016, p. e0167503. Pubmed, doi:10.1371/journal.pone.0167503.
Tian X, Patel K, Ridpath JR, Chen Y, Zhou Y-H, Neo D, Clement J, Takata M, Takeda S, Sale J, Wright FA, Swenberg JA, Nakamura J. Homologous Recombination and Translesion DNA Synthesis Play Critical Roles on Tolerating DNA Damage Caused by Trace Levels of Hexavalent Chromium. PLoS One. 2016;11(12):e0167503.

Published In

PLoS One

DOI

EISSN

1932-6203

Publication Date

2016

Volume

11

Issue

12

Start / End Page

e0167503

Location

United States

Related Subject Headings

  • Time Factors
  • Mutation
  • Humans
  • Homologous Recombination
  • General Science & Technology
  • Dose-Response Relationship, Drug
  • DNA Replication
  • DNA Damage
  • Chromium
  • Chickens