Prion disease diagnosis by proteomic profiling.
Publication
, Journal Article
Herbst, A; McIlwain, S; Schmidt, JJ; Aiken, JM; Page, CD; Li, L
Published in: J Proteome Res
February 2009
Definitive prion disease diagnosis is currently limited to postmortem assay for the presence of the disease-associated proteinase K-resistant prion protein. Using cerebrospinal fluid (CSF) from prion-infected hamsters, matrix-assisted laser desorption/ionization Fourier transform mass spectrometry (MALDI-FTMS), and support vector machines (SVM), we have identified peptide profiles characteristic of disease state. Using 10-fold leave-one-out cross-validation, we report a predictive accuracy of 72% with a true positive rate of 73% and a false positive rate of 27% demonstrating the suitability of using proteomic profiling and CSF for the development of multiple marker diagnostics of prion disease.
Duke Scholars
Published In
J Proteome Res
DOI
ISSN
1535-3893
Publication Date
February 2009
Volume
8
Issue
2
Start / End Page
1030 / 1036
Location
United States
Related Subject Headings
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
- Sensitivity and Specificity
- Reproducibility of Results
- Protein Array Analysis
- Prions
- Prion Diseases
- Humans
- Cricetinae
- Biomarkers
- Biochemistry & Molecular Biology
Citation
APA
Chicago
ICMJE
MLA
NLM
Herbst, A., McIlwain, S., Schmidt, J. J., Aiken, J. M., Page, C. D., & Li, L. (2009). Prion disease diagnosis by proteomic profiling. J Proteome Res, 8(2), 1030–1036. https://doi.org/10.1021/pr800832s
Herbst, Allen, Sean McIlwain, Joshua J. Schmidt, Judd M. Aiken, C David Page, and Lingjun Li. “Prion disease diagnosis by proteomic profiling.” J Proteome Res 8, no. 2 (February 2009): 1030–36. https://doi.org/10.1021/pr800832s.
Herbst A, McIlwain S, Schmidt JJ, Aiken JM, Page CD, Li L. Prion disease diagnosis by proteomic profiling. J Proteome Res. 2009 Feb;8(2):1030–6.
Herbst, Allen, et al. “Prion disease diagnosis by proteomic profiling.” J Proteome Res, vol. 8, no. 2, Feb. 2009, pp. 1030–36. Pubmed, doi:10.1021/pr800832s.
Herbst A, McIlwain S, Schmidt JJ, Aiken JM, Page CD, Li L. Prion disease diagnosis by proteomic profiling. J Proteome Res. 2009 Feb;8(2):1030–1036.
Published In
J Proteome Res
DOI
ISSN
1535-3893
Publication Date
February 2009
Volume
8
Issue
2
Start / End Page
1030 / 1036
Location
United States
Related Subject Headings
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
- Sensitivity and Specificity
- Reproducibility of Results
- Protein Array Analysis
- Prions
- Prion Diseases
- Humans
- Cricetinae
- Biomarkers
- Biochemistry & Molecular Biology