Characterization of Acyl Carrier Protein-Dependent Glycosyltransferase in Mitomycin C Biosynthesis.
Publication
, Journal Article
Nguyen, HP; Yokoyama, K
Published in: Biochemistry
June 25, 2019
Mitomycins make up a group of antitumor natural products that are biosynthesized from aminohydroxybenzoic acid (AHBA) and N-acetylglucosamine (GlcNAc). While the biosynthetic gene cluster was reported two decades ago, the mechanism by which the two building blocks, AHBA and GlcNAc, are coupled during biosynthesis remained uncharacterized. Here we report evidence that AHBA is first loaded onto an MmcB acyl carrier protein (ACP) by a MitE acyl ACP synthetase, followed by a transfer of GlcNAc from UDP-GlcNAc by MitB. The results suggest that the early steps of mitomycin biosynthesis proceed via intermediates linked to MmcB.
Duke Scholars
Published In
Biochemistry
DOI
EISSN
1520-4995
Publication Date
June 25, 2019
Volume
58
Issue
25
Start / End Page
2804 / 2808
Location
United States
Related Subject Headings
- Streptomyces
- N-Acetylglucosaminyltransferases
- Mitomycin
- Kinetics
- Hydroxybenzoates
- Enzyme Assays
- Carbon-Sulfur Ligases
- Biochemistry & Molecular Biology
- Aminobenzoates
- Acyl Carrier Protein
Citation
APA
Chicago
ICMJE
MLA
NLM
Nguyen, H. P., & Yokoyama, K. (2019). Characterization of Acyl Carrier Protein-Dependent Glycosyltransferase in Mitomycin C Biosynthesis. Biochemistry, 58(25), 2804–2808. https://doi.org/10.1021/acs.biochem.9b00379
Nguyen, Hai P., and Kenichi Yokoyama. “Characterization of Acyl Carrier Protein-Dependent Glycosyltransferase in Mitomycin C Biosynthesis.” Biochemistry 58, no. 25 (June 25, 2019): 2804–8. https://doi.org/10.1021/acs.biochem.9b00379.
Nguyen HP, Yokoyama K. Characterization of Acyl Carrier Protein-Dependent Glycosyltransferase in Mitomycin C Biosynthesis. Biochemistry. 2019 Jun 25;58(25):2804–8.
Nguyen, Hai P., and Kenichi Yokoyama. “Characterization of Acyl Carrier Protein-Dependent Glycosyltransferase in Mitomycin C Biosynthesis.” Biochemistry, vol. 58, no. 25, June 2019, pp. 2804–08. Pubmed, doi:10.1021/acs.biochem.9b00379.
Nguyen HP, Yokoyama K. Characterization of Acyl Carrier Protein-Dependent Glycosyltransferase in Mitomycin C Biosynthesis. Biochemistry. 2019 Jun 25;58(25):2804–2808.
Published In
Biochemistry
DOI
EISSN
1520-4995
Publication Date
June 25, 2019
Volume
58
Issue
25
Start / End Page
2804 / 2808
Location
United States
Related Subject Headings
- Streptomyces
- N-Acetylglucosaminyltransferases
- Mitomycin
- Kinetics
- Hydroxybenzoates
- Enzyme Assays
- Carbon-Sulfur Ligases
- Biochemistry & Molecular Biology
- Aminobenzoates
- Acyl Carrier Protein