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Epicutaneous sensitization results in IgE-dependent intestinal mast cell expansion and food-induced anaphylaxis.

Publication ,  Journal Article
Bartnikas, LM; Gurish, MF; Burton, OT; Leisten, S; Janssen, E; Oettgen, HC; Beaupré, J; Lewis, CN; Austen, KF; Schulte, S; Hornick, JL ...
Published in: The Journal of allergy and clinical immunology
February 2013

Sensitization to food antigen can occur through cutaneous exposure.We sought to test the hypothesis that epicutaneous sensitization with food antigen predisposes to IgE-mediated anaphylaxis on oral allergen challenge.BALB/c mice were epicutaneously sensitized by repeated application of ovalbumin (OVA) to tape-stripped skin over 7 weeks or orally immunized with OVA and cholera toxin (CT) weekly for 8 weeks and then orally challenged with OVA. Body temperature was monitored, and serum mouse mast cell protease 1 levels were determined after challenge. Tissue mast cell (MC) counts were examined by using chloroacetate esterase staining. Levels of serum OVA-specific IgE and IgG(1) antibodies and cytokines in supernatants of OVA-stimulated splenocytes were measured by means of ELISA. Serum IL-4 levels were measured by using an in vivo cytokine capture assay.Epicutaneously sensitized mice exhibited expansion of connective tissue MCs in the jejunum, increased serum IL-4 levels, and systemic anaphylaxis after oral challenge, as evidenced by decreased body temperature and increased serum mouse mast cell protease 1 levels. Intestinal MC expansion and anaphylaxis were IgE dependent because they did not occur in epicutaneously sensitized IgE(-/-) mice. Mice orally immunized with OVA plus CT did not have increased serum IL-4 levels, expanded intestinal MCs, or anaphylaxis after oral challenge, despite OVA-specific IgE levels and splenocyte cytokine production in response to OVA stimulation, which were comparable with those of epicutaneously sensitized mice.Epicutaneously sensitized mice, but not mice orally immunized with antigen plus CT, have expansion of intestinal MCs and IgE-mediated anaphylaxis after single oral antigen challenge. IgE is necessary but not sufficient for food anaphylaxis, and MC expansion in the gut can play an important role in the development of anaphylaxis.

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Published In

The Journal of allergy and clinical immunology

DOI

EISSN

1097-6825

ISSN

0091-6749

Publication Date

February 2013

Volume

131

Issue

2

Start / End Page

451-60.e1-6

Related Subject Headings

  • Skin
  • Ovalbumin
  • Mice, Inbred BALB C
  • Mice
  • Mast Cells
  • Jejunum
  • Interleukin-4
  • Immunoglobulin G
  • Immunoglobulin E
  • Food Hypersensitivity
 

Citation

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Bartnikas, L. M., Gurish, M. F., Burton, O. T., Leisten, S., Janssen, E., Oettgen, H. C., … Oyoshi, M. K. (2013). Epicutaneous sensitization results in IgE-dependent intestinal mast cell expansion and food-induced anaphylaxis. The Journal of Allergy and Clinical Immunology, 131(2), 451-60.e1-6. https://doi.org/10.1016/j.jaci.2012.11.032
Bartnikas, Lisa M., Michael F. Gurish, Oliver T. Burton, Sabine Leisten, Erin Janssen, Hans C. Oettgen, Jacqueline Beaupré, et al. “Epicutaneous sensitization results in IgE-dependent intestinal mast cell expansion and food-induced anaphylaxis.The Journal of Allergy and Clinical Immunology 131, no. 2 (February 2013): 451-60.e1-6. https://doi.org/10.1016/j.jaci.2012.11.032.
Bartnikas LM, Gurish MF, Burton OT, Leisten S, Janssen E, Oettgen HC, et al. Epicutaneous sensitization results in IgE-dependent intestinal mast cell expansion and food-induced anaphylaxis. The Journal of allergy and clinical immunology. 2013 Feb;131(2):451-60.e1-6.
Bartnikas, Lisa M., et al. “Epicutaneous sensitization results in IgE-dependent intestinal mast cell expansion and food-induced anaphylaxis.The Journal of Allergy and Clinical Immunology, vol. 131, no. 2, Feb. 2013, pp. 451-60.e1-6. Epmc, doi:10.1016/j.jaci.2012.11.032.
Bartnikas LM, Gurish MF, Burton OT, Leisten S, Janssen E, Oettgen HC, Beaupré J, Lewis CN, Austen KF, Schulte S, Hornick JL, Geha RS, Oyoshi MK. Epicutaneous sensitization results in IgE-dependent intestinal mast cell expansion and food-induced anaphylaxis. The Journal of allergy and clinical immunology. 2013 Feb;131(2):451-60.e1–6.
Journal cover image

Published In

The Journal of allergy and clinical immunology

DOI

EISSN

1097-6825

ISSN

0091-6749

Publication Date

February 2013

Volume

131

Issue

2

Start / End Page

451-60.e1-6

Related Subject Headings

  • Skin
  • Ovalbumin
  • Mice, Inbred BALB C
  • Mice
  • Mast Cells
  • Jejunum
  • Interleukin-4
  • Immunoglobulin G
  • Immunoglobulin E
  • Food Hypersensitivity