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Measurement of methotrexate polyglutamates in human erythrocytes by ion-pair UPLC-MS/MS.

Publication ,  Journal Article
van Haandel, L; Becker, ML; Williams, TD; Leeder, JS; Stobaugh, JF
Published in: Bioanalysis
December 2011

BACKGROUND: Low-dose methotrexate is used for the treatment of rheumatoid arthritis and juvenile idiopathic arthritis, but its effectiveness greatly varies between individuals. Therapeutic drug monitoring of intracellular methotrexate metabolites, the γ-polyglutamates (MTXGlu(n)), in human erythrocytes has shown promise in providing a basis for individualization of therapy. RESULTS: This work presents expedient methodology for the analysis of MTXGlu(1-7) in human erythrocytes by ion-pair UPLC with detection by tandem MS (UPLC-ESI-MS/MS). The use of N,N-dimethylheptylamine as an ion-pair agent was found to be favorable over others. Thermal extraction of erythrocyte lysates provides a simple one-step extraction procedure. The entire chromatographic run time is 6 min and the assay was validated within the therapeutic range of these metabolites CONCLUSION: The developed sample preparation procedure in combination with ion-pair UPLC-ESI-MS/MS analysis allowed for expedient quantitation of MTXGlu(1-7) in human erythrocytes. The rapid analysis time would enable therapeutic drug monitoring of MTXGlu(1-7) in the clinic.

Duke Scholars

Published In

Bioanalysis

DOI

EISSN

1757-6199

Publication Date

December 2011

Volume

3

Issue

24

Start / End Page

2783 / 2796

Location

England

Related Subject Headings

  • Tandem Mass Spectrometry
  • Reproducibility of Results
  • Polyglutamic Acid
  • Phenethylamines
  • Methotrexate
  • Isotope Labeling
  • Ions
  • Humans
  • Erythrocytes
  • Deuterium
 

Citation

APA
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ICMJE
MLA
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van Haandel, L., Becker, M. L., Williams, T. D., Leeder, J. S., & Stobaugh, J. F. (2011). Measurement of methotrexate polyglutamates in human erythrocytes by ion-pair UPLC-MS/MS. Bioanalysis, 3(24), 2783–2796. https://doi.org/10.4155/bio.11.288
Haandel, Leon van, Mara L. Becker, T. D. Williams, J Steven Leeder, and John F. Stobaugh. “Measurement of methotrexate polyglutamates in human erythrocytes by ion-pair UPLC-MS/MS.Bioanalysis 3, no. 24 (December 2011): 2783–96. https://doi.org/10.4155/bio.11.288.
van Haandel L, Becker ML, Williams TD, Leeder JS, Stobaugh JF. Measurement of methotrexate polyglutamates in human erythrocytes by ion-pair UPLC-MS/MS. Bioanalysis. 2011 Dec;3(24):2783–96.
van Haandel, Leon, et al. “Measurement of methotrexate polyglutamates in human erythrocytes by ion-pair UPLC-MS/MS.Bioanalysis, vol. 3, no. 24, Dec. 2011, pp. 2783–96. Pubmed, doi:10.4155/bio.11.288.
van Haandel L, Becker ML, Williams TD, Leeder JS, Stobaugh JF. Measurement of methotrexate polyglutamates in human erythrocytes by ion-pair UPLC-MS/MS. Bioanalysis. 2011 Dec;3(24):2783–2796.
Journal cover image

Published In

Bioanalysis

DOI

EISSN

1757-6199

Publication Date

December 2011

Volume

3

Issue

24

Start / End Page

2783 / 2796

Location

England

Related Subject Headings

  • Tandem Mass Spectrometry
  • Reproducibility of Results
  • Polyglutamic Acid
  • Phenethylamines
  • Methotrexate
  • Isotope Labeling
  • Ions
  • Humans
  • Erythrocytes
  • Deuterium