Skip to main content
Journal cover image

On Command Drug Delivery via Cell-Conveyed Phototherapeutics.

Publication ,  Journal Article
Marvin, CM; Ding, S; White, RE; Orlova, N; Wang, Q; Zywot, EM; Vickerman, BM; Harr, L; Tarrant, TK; Dayton, PA; Lawrence, DS
Published in: Small
September 2019

Herein, the use of red blood cells (RBCs) as carriers of cytoplasmically interned phototherapeutic agents is described. Photolysis promotes drug release from the RBC carrier thereby providing the means to target specific diseased sites. This strategy is realized with a vitamin B12-taxane conjugate (B12-TAX), in which the drug is linked to the vitamin via a photolabile CoC bond. The conjugate is introduced into mouse RBCs (mRBCs) via a pore-forming/pore-resealing procedure and is cytoplasmically retained due to the membrane impermeability of B12. Photolysis separates the taxane from the B12 cytoplasmic anchor, enabling the drug to exit the RBC carrier. A covalently appended Cy5 antenna sensitizes the conjugate (Cy5-B12-TAX) to far red light, thereby circumventing the intense light absorbing properties of hemoglobin (350-600 nm). Microscopy and imaging flow cytometry reveal that Cy5-B12-TAX-loaded mRBCs act as drug carriers. Furthermore, intravital imaging of mice furnish a real time assessment of circulating phototherapeutic-loaded mRBCs as well as evidence of the targeted photorelease of the taxane upon photolysis. Histopathology confirms that drug release occurs in a well resolved spatiotemporal fashion. Finally, acoustic angiography is employed to assess the consequences of taxane release at the tumor site in Nu/Nu-tumor-bearing mice.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Small

DOI

EISSN

1613-6829

Publication Date

September 2019

Volume

15

Issue

37

Start / End Page

e1901442

Location

Germany

Related Subject Headings

  • Vitamin B 12
  • Taxoids
  • Prodrugs
  • Photolysis
  • Nanoscience & Nanotechnology
  • Mice
  • Humans
  • Flow Cytometry
  • Female
  • Erythrocytes
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Marvin, C. M., Ding, S., White, R. E., Orlova, N., Wang, Q., Zywot, E. M., … Lawrence, D. S. (2019). On Command Drug Delivery via Cell-Conveyed Phototherapeutics. Small, 15(37), e1901442. https://doi.org/10.1002/smll.201901442
Marvin, Christina M., Song Ding, Rachel E. White, Natalia Orlova, Qunzhao Wang, Emilia M. Zywot, Brianna M. Vickerman, et al. “On Command Drug Delivery via Cell-Conveyed Phototherapeutics.Small 15, no. 37 (September 2019): e1901442. https://doi.org/10.1002/smll.201901442.
Marvin CM, Ding S, White RE, Orlova N, Wang Q, Zywot EM, et al. On Command Drug Delivery via Cell-Conveyed Phototherapeutics. Small. 2019 Sep;15(37):e1901442.
Marvin, Christina M., et al. “On Command Drug Delivery via Cell-Conveyed Phototherapeutics.Small, vol. 15, no. 37, Sept. 2019, p. e1901442. Pubmed, doi:10.1002/smll.201901442.
Marvin CM, Ding S, White RE, Orlova N, Wang Q, Zywot EM, Vickerman BM, Harr L, Tarrant TK, Dayton PA, Lawrence DS. On Command Drug Delivery via Cell-Conveyed Phototherapeutics. Small. 2019 Sep;15(37):e1901442.
Journal cover image

Published In

Small

DOI

EISSN

1613-6829

Publication Date

September 2019

Volume

15

Issue

37

Start / End Page

e1901442

Location

Germany

Related Subject Headings

  • Vitamin B 12
  • Taxoids
  • Prodrugs
  • Photolysis
  • Nanoscience & Nanotechnology
  • Mice
  • Humans
  • Flow Cytometry
  • Female
  • Erythrocytes