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Data from: In vivo pump-probe and multiphoton fluorescence microscopy of melanoma and pigmented lesions in a mouse model

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Fischer, M; Wilson, J; Degan, S; Warren, W; Zhang, J; Mitropoulos, T; Gainey, C; Simpson, MJ
July 15, 2019

We demonstrate a multimodal approach that combines a pump--probe with confocal reflectance and multiphoton autofluorescence microscopy. Pump--probe microscopy has been proven to be of great value in analyzing thin tissue sections of pigmented lesions, as it produces molecular contrast which is inaccessible by other means. However, the higher optical intensity required to overcome scattering in thick tissue leads to higher-order nonlinearities in the optical response of melanin (e.g., two-photon pump and one-photon probe) that present additional challenges for interpreting the data. We show that analysis of pigment composition in vivo must carefully account for signal terms that are nonlinear with respect to the pump and probe intensities. We find that pump--probe imaging gives useful contrast for pigmented structures over a large range of spatial scales (100 μm to 1 cm), making it a potentially useful tool for tracking the progression of pigmented lesions without the need to introduce exogenous contrast agents.

Duke Scholars

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Publication Date

July 15, 2019
 

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Fischer, M., Wilson, J., Degan, S., Warren, W., Zhang, J., Mitropoulos, T., … Simpson, M. J. (2019). Data from: In vivo pump-probe and multiphoton fluorescence microscopy of melanoma and pigmented lesions in a mouse model. https://doi.org/10.7924/r4cc0zp95
Fischer, Martin, Jesse Wilson, Simone Degan, Warren Warren, Jennifer Zhang, Tanya Mitropoulos, Christina Gainey, and Mary Jane Simpson. “Data from: In vivo pump-probe and multiphoton fluorescence microscopy of melanoma and pigmented lesions in a mouse model,” July 15, 2019. https://doi.org/10.7924/r4cc0zp95.
Fischer M, Wilson J, Degan S, Warren W, Zhang J, Mitropoulos T, Gainey C, Simpson MJ. Data from: In vivo pump-probe and multiphoton fluorescence microscopy of melanoma and pigmented lesions in a mouse model. 2019.

DOI

Publication Date

July 15, 2019