Forward and Reverse Genetic Analysis of Chlamydia.
Publication
, Chapter
Kędzior, M; Bastidas, RJ
2019
Chlamydia is a major etiological agent of human disease that affects millions of individuals worldwide. Historically, our understanding of the mechanisms that contribute to its pathogenesis has been limited. However, the recent development of powerful genetic tools for manipulating Chlamydia has resulted in significant gains in our ability to dissect its virulence mechanisms. These tools have overcome several barriers for manipulating intracellular pathogens and are amenable for the routine genetic engineering of Chlamydia. Here, we provide several detailed protocols for performing genetic analysis in Chlamydia trachomatis allowing investigators to elucidate how this obligate intracellular pathogen causes disease.
Duke Scholars
DOI
Publication Date
2019
Volume
2042
Start / End Page
185 / 204
Related Subject Headings
- Vero Cells
- Reverse Genetics
- Polymorphism, Single Nucleotide
- Mutagenesis
- Humans
- Genetic Linkage
- Developmental Biology
- DNA, Bacterial
- Chlorocebus aethiops
- Chlamydia trachomatis
Citation
APA
Chicago
ICMJE
MLA
NLM
Kędzior, M., & Bastidas, R. J. (2019). Forward and Reverse Genetic Analysis of Chlamydia. (Vol. 2042, pp. 185–204). https://doi.org/10.1007/978-1-4939-9694-0_13
Kędzior, Mateusz, and Robert J. Bastidas. “Forward and Reverse Genetic Analysis of Chlamydia.,” 2042:185–204, 2019. https://doi.org/10.1007/978-1-4939-9694-0_13.
Kędzior M, Bastidas RJ. Forward and Reverse Genetic Analysis of Chlamydia. In 2019. p. 185–204.
Kędzior, Mateusz, and Robert J. Bastidas. Forward and Reverse Genetic Analysis of Chlamydia. Vol. 2042, 2019, pp. 185–204. Pubmed, doi:10.1007/978-1-4939-9694-0_13.
Kędzior M, Bastidas RJ. Forward and Reverse Genetic Analysis of Chlamydia. 2019. p. 185–204.
DOI
Publication Date
2019
Volume
2042
Start / End Page
185 / 204
Related Subject Headings
- Vero Cells
- Reverse Genetics
- Polymorphism, Single Nucleotide
- Mutagenesis
- Humans
- Genetic Linkage
- Developmental Biology
- DNA, Bacterial
- Chlorocebus aethiops
- Chlamydia trachomatis