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Forward and Reverse Genetic Analysis of Chlamydia.

Publication ,  Chapter
Kędzior, M; Bastidas, RJ
2019

Chlamydia is a major etiological agent of human disease that affects millions of individuals worldwide. Historically, our understanding of the mechanisms that contribute to its pathogenesis has been limited. However, the recent development of powerful genetic tools for manipulating Chlamydia has resulted in significant gains in our ability to dissect its virulence mechanisms. These tools have overcome several barriers for manipulating intracellular pathogens and are amenable for the routine genetic engineering of Chlamydia. Here, we provide several detailed protocols for performing genetic analysis in Chlamydia trachomatis allowing investigators to elucidate how this obligate intracellular pathogen causes disease.

Duke Scholars

DOI

Publication Date

2019

Volume

2042

Start / End Page

185 / 204

Related Subject Headings

  • Vero Cells
  • Reverse Genetics
  • Polymorphism, Single Nucleotide
  • Mutagenesis
  • Humans
  • Genetic Linkage
  • Developmental Biology
  • DNA, Bacterial
  • Chlorocebus aethiops
  • Chlamydia trachomatis
 

Citation

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Kędzior, M., & Bastidas, R. J. (2019). Forward and Reverse Genetic Analysis of Chlamydia. (Vol. 2042, pp. 185–204). https://doi.org/10.1007/978-1-4939-9694-0_13
Kędzior, Mateusz, and Robert J. Bastidas. “Forward and Reverse Genetic Analysis of Chlamydia.,” 2042:185–204, 2019. https://doi.org/10.1007/978-1-4939-9694-0_13.
Kędzior M, Bastidas RJ. Forward and Reverse Genetic Analysis of Chlamydia. In 2019. p. 185–204.
Kędzior, Mateusz, and Robert J. Bastidas. Forward and Reverse Genetic Analysis of Chlamydia. Vol. 2042, 2019, pp. 185–204. Pubmed, doi:10.1007/978-1-4939-9694-0_13.
Kędzior M, Bastidas RJ. Forward and Reverse Genetic Analysis of Chlamydia. 2019. p. 185–204.

DOI

Publication Date

2019

Volume

2042

Start / End Page

185 / 204

Related Subject Headings

  • Vero Cells
  • Reverse Genetics
  • Polymorphism, Single Nucleotide
  • Mutagenesis
  • Humans
  • Genetic Linkage
  • Developmental Biology
  • DNA, Bacterial
  • Chlorocebus aethiops
  • Chlamydia trachomatis