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Monitoring changes in gene expression in renal ischemia-reperfusion in the rat.

Publication ,  Journal Article
Yoshida, T; Kurella, M; Beato, F; Min, H; Ingelfinger, JR; Stears, RL; Swinford, RD; Gullans, SR; Tang, S-S
Published in: Kidney Int
May 2002

BACKGROUND: Although acute renal failure (ARF) is a relatively common disorder with major morbidity and mortality, its molecular basis remains incompletely defined. The present study examined global gene expression in the well-characterized ischemia-reperfusion model of ARF using DNA microarray technology. METHODS: Male Wistar rats underwent bilateral renal ischemia (30 min) or sham operation, followed by reperfusion for 1, 2, 3 or 4 days. Plasma creatinine increased approximately fivefold over baseline, peaking on day 1. Renal total RNA was used to probe cDNA microarrays. RESULTS: Alterations in expression of 18 genes were identified by microarray analysis. Nine genes were up-regulated (ADAM2, HO-1, UCP-2, and thymosin beta4 in the early phase and clusterin, vanin1, fibronectin, heat-responsive protein 12 and FK506 binding protein in the established phase), whereas another nine were down-regulated (glutamine synthetase, cytochrome p450 IId6, and cyp 2d9 in the early phase and cyp 4a14, Xist gene, PPARgamma, alpha-albumin, uromodulin, and ADH B2 in the established phase). The identities of these 18 genes were sequence-verified. Changes in gene expression of ADAM2, cyp2d6, fibronectin, HO-1 and PPARgamma were confirmed by quantitative real-time polymerase chain reaction (PCR). ADAM2, cyp2d6, and PPARgamma have not previously been known to be involved in ARF. CONCLUSION: Using DNA microarray technology, we identified changes in expression of 18 genes during renal ischemia-reperfusion injury in the rat. We confirmed changes in five genes (fibronectin, ADAM2, cyp 2d6, HO-1 and PPARgamma) by quantitative real-time PCR. Several genes, not previously been identified as playing a role in ischemic ARF, may have importance in this disease.

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Published In

Kidney Int

DOI

ISSN

0085-2538

Publication Date

May 2002

Volume

61

Issue

5

Start / End Page

1646 / 1654

Location

United States

Related Subject Headings

  • Urology & Nephrology
  • Up-Regulation
  • Transcription Factors
  • Reperfusion Injury
  • Receptors, Cytoplasmic and Nuclear
  • Rats, Wistar
  • Rats
  • Oligonucleotide Array Sequence Analysis
  • Metalloendopeptidases
  • Membrane Glycoproteins
 

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Yoshida, T., Kurella, M., Beato, F., Min, H., Ingelfinger, J. R., Stears, R. L., … Tang, S.-S. (2002). Monitoring changes in gene expression in renal ischemia-reperfusion in the rat. Kidney Int, 61(5), 1646–1654. https://doi.org/10.1046/j.1523-1755.2002.00341.x
Yoshida, Takumi, Manjula Kurella, Francisca Beato, Hyunsuk Min, Julie R. Ingelfinger, Robin L. Stears, Rita D. Swinford, Steven R. Gullans, and Shiow-Shih Tang. “Monitoring changes in gene expression in renal ischemia-reperfusion in the rat.Kidney Int 61, no. 5 (May 2002): 1646–54. https://doi.org/10.1046/j.1523-1755.2002.00341.x.
Yoshida T, Kurella M, Beato F, Min H, Ingelfinger JR, Stears RL, et al. Monitoring changes in gene expression in renal ischemia-reperfusion in the rat. Kidney Int. 2002 May;61(5):1646–54.
Yoshida, Takumi, et al. “Monitoring changes in gene expression in renal ischemia-reperfusion in the rat.Kidney Int, vol. 61, no. 5, May 2002, pp. 1646–54. Pubmed, doi:10.1046/j.1523-1755.2002.00341.x.
Yoshida T, Kurella M, Beato F, Min H, Ingelfinger JR, Stears RL, Swinford RD, Gullans SR, Tang S-S. Monitoring changes in gene expression in renal ischemia-reperfusion in the rat. Kidney Int. 2002 May;61(5):1646–1654.
Journal cover image

Published In

Kidney Int

DOI

ISSN

0085-2538

Publication Date

May 2002

Volume

61

Issue

5

Start / End Page

1646 / 1654

Location

United States

Related Subject Headings

  • Urology & Nephrology
  • Up-Regulation
  • Transcription Factors
  • Reperfusion Injury
  • Receptors, Cytoplasmic and Nuclear
  • Rats, Wistar
  • Rats
  • Oligonucleotide Array Sequence Analysis
  • Metalloendopeptidases
  • Membrane Glycoproteins