Skip to main content
Journal cover image

Magnetoelectroporation: improved labeling of neural stem cells and leukocytes for cellular magnetic resonance imaging using a single FDA-approved agent.

Publication ,  Journal Article
Walczak, P; Ruiz-Cabello, J; Kedziorek, DA; Gilad, AA; Lin, S; Barnett, B; Qin, L; Levitsky, H; Bulte, JWM
Published in: Nanomedicine : nanotechnology, biology, and medicine
June 2006

Cellular magnetic resonance imaging (MRI) relies on the use of intracellular contrast agents, primarily iron oxide compounds. Several techniques have been used to efficiently shuttle iron oxides into nonphagocytic cells, but all methods used until now require a prolonged incubation of cells. We hypothesized that instant magnetic labeling of cells could be achieved using electroporation. Neural stem cells (NSCs) and leukocytes from spleen and lymph nodes were suspended in a ferumoxide labeling solution, loaded into cuvettes, and subjected to electromechanical permeabilization using electroporation. Magnetically labeled cells were assayed for labeling efficiency, as well as for potential toxicity or altered function. To confirm the method's applicability to detect cells, MRI experiments were performed at 11.7 T. Magnetoelectroporation of NSCs, as demonstrated by Prussian blue staining, anti-dextran immunostaining, and a quantitative iron uptake assay, proved to be an efficient intracellular magnetic labeling method. Leukocytes including lymphocytes, which are notoriously difficult to label because of their membrane properties and small cytoplasmic volume, also demonstrated a pronounced uptake of ferumoxide. MRI experiments showed that labeled NSCs could be visualized as single cells and cell clusters in gelatin phantoms, and as proliferating cell masses in mouse brain. We have developed a convenient technique for instant magnetic labeling of cells. Because magnetoelectroporation allows the use of ferumoxides approved by the US Food and Drug Administration without additional agents, it has excellent potential for clinical translation.

Published In

Nanomedicine : nanotechnology, biology, and medicine

DOI

EISSN

1549-9642

ISSN

1549-9634

Publication Date

June 2006

Volume

2

Issue

2

Start / End Page

89 / 94

Related Subject Headings

  • United States Food and Drug Administration
  • United States
  • Stem Cells
  • Neurons
  • Nanoscience & Nanotechnology
  • Mice
  • Magnetics
  • Magnetic Resonance Imaging
  • Leukocytes
  • Electroporation
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Walczak, P., Ruiz-Cabello, J., Kedziorek, D. A., Gilad, A. A., Lin, S., Barnett, B., … Bulte, J. W. M. (2006). Magnetoelectroporation: improved labeling of neural stem cells and leukocytes for cellular magnetic resonance imaging using a single FDA-approved agent. Nanomedicine : Nanotechnology, Biology, and Medicine, 2(2), 89–94. https://doi.org/10.1016/j.nano.2006.01.003
Walczak, Piotr, Jesus Ruiz-Cabello, Dorota A. Kedziorek, Assaf A. Gilad, Sopo Lin, Bradley Barnett, Lu Qin, Hyam Levitsky, and Jeff W. M. Bulte. “Magnetoelectroporation: improved labeling of neural stem cells and leukocytes for cellular magnetic resonance imaging using a single FDA-approved agent.Nanomedicine : Nanotechnology, Biology, and Medicine 2, no. 2 (June 2006): 89–94. https://doi.org/10.1016/j.nano.2006.01.003.
Walczak P, Ruiz-Cabello J, Kedziorek DA, Gilad AA, Lin S, Barnett B, et al. Magnetoelectroporation: improved labeling of neural stem cells and leukocytes for cellular magnetic resonance imaging using a single FDA-approved agent. Nanomedicine : nanotechnology, biology, and medicine. 2006 Jun;2(2):89–94.
Walczak, Piotr, et al. “Magnetoelectroporation: improved labeling of neural stem cells and leukocytes for cellular magnetic resonance imaging using a single FDA-approved agent.Nanomedicine : Nanotechnology, Biology, and Medicine, vol. 2, no. 2, June 2006, pp. 89–94. Epmc, doi:10.1016/j.nano.2006.01.003.
Walczak P, Ruiz-Cabello J, Kedziorek DA, Gilad AA, Lin S, Barnett B, Qin L, Levitsky H, Bulte JWM. Magnetoelectroporation: improved labeling of neural stem cells and leukocytes for cellular magnetic resonance imaging using a single FDA-approved agent. Nanomedicine : nanotechnology, biology, and medicine. 2006 Jun;2(2):89–94.
Journal cover image

Published In

Nanomedicine : nanotechnology, biology, and medicine

DOI

EISSN

1549-9642

ISSN

1549-9634

Publication Date

June 2006

Volume

2

Issue

2

Start / End Page

89 / 94

Related Subject Headings

  • United States Food and Drug Administration
  • United States
  • Stem Cells
  • Neurons
  • Nanoscience & Nanotechnology
  • Mice
  • Magnetics
  • Magnetic Resonance Imaging
  • Leukocytes
  • Electroporation