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Cannabis use is associated with potentially heritable widespread changes in autism candidate gene DLGAP2 DNA methylation in sperm.

Publication ,  Journal Article
Schrott, R; Acharya, K; Itchon-Ramos, N; Hawkey, AB; Pippen, E; Mitchell, JT; Kollins, SH; Levin, ED; Murphy, SK
Published in: Epigenetics
2020

Parental cannabis use has been associated with adverse neurodevelopmental outcomes in offspring, but how such phenotypes are transmitted is largely unknown. Using reduced representation bisulphite sequencing (RRBS), we recently demonstrated that cannabis use is associated with widespread DNA methylation changes in human and rat sperm. Discs-Large Associated Protein 2 (DLGAP2), involved in synapse organization, neuronal signaling, and strongly implicated in autism, exhibited significant hypomethylation (p < 0.05) at 17 CpG sites in human sperm. We successfully validated the differential methylation present in DLGAP2 for nine CpG sites located in intron seven (p < 0.05) using quantitative bisulphite pyrosequencing. Intron 7 DNA methylation and DLGAP2 expression in human conceptal brain tissue were inversely correlated (p < 0.01). Adult male rats exposed to delta-9-tetrahydrocannabinol (THC) showed differential DNA methylation at Dlgap2 in sperm (p < 0.03), as did the nucleus accumbens of rats whose fathers were exposed to THC prior to conception (p < 0.05). Altogether, these results warrant further investigation into the effects of preconception cannabis use in males and the potential effects on subsequent generations.

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Published In

Epigenetics

DOI

EISSN

1559-2308

Publication Date

2020

Volume

15

Issue

1-2

Start / End Page

161 / 173

Location

United States

Related Subject Headings

  • Spermatozoa
  • Rats, Sprague-Dawley
  • Rats
  • Nucleus Accumbens
  • Nerve Tissue Proteins
  • Marijuana Abuse
  • Male
  • Introns
  • Humans
  • Dronabinol
 

Citation

APA
Chicago
ICMJE
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Schrott, R., Acharya, K., Itchon-Ramos, N., Hawkey, A. B., Pippen, E., Mitchell, J. T., … Murphy, S. K. (2020). Cannabis use is associated with potentially heritable widespread changes in autism candidate gene DLGAP2 DNA methylation in sperm. Epigenetics, 15(1–2), 161–173. https://doi.org/10.1080/15592294.2019.1656158
Schrott, Rose, Kelly Acharya, Nilda Itchon-Ramos, Andrew B. Hawkey, Erica Pippen, John T. Mitchell, Scott H. Kollins, Edward D. Levin, and Susan K. Murphy. “Cannabis use is associated with potentially heritable widespread changes in autism candidate gene DLGAP2 DNA methylation in sperm.Epigenetics 15, no. 1–2 (2020): 161–73. https://doi.org/10.1080/15592294.2019.1656158.
Schrott R, Acharya K, Itchon-Ramos N, Hawkey AB, Pippen E, Mitchell JT, et al. Cannabis use is associated with potentially heritable widespread changes in autism candidate gene DLGAP2 DNA methylation in sperm. Epigenetics. 2020;15(1–2):161–73.
Schrott, Rose, et al. “Cannabis use is associated with potentially heritable widespread changes in autism candidate gene DLGAP2 DNA methylation in sperm.Epigenetics, vol. 15, no. 1–2, 2020, pp. 161–73. Pubmed, doi:10.1080/15592294.2019.1656158.
Schrott R, Acharya K, Itchon-Ramos N, Hawkey AB, Pippen E, Mitchell JT, Kollins SH, Levin ED, Murphy SK. Cannabis use is associated with potentially heritable widespread changes in autism candidate gene DLGAP2 DNA methylation in sperm. Epigenetics. 2020;15(1–2):161–173.

Published In

Epigenetics

DOI

EISSN

1559-2308

Publication Date

2020

Volume

15

Issue

1-2

Start / End Page

161 / 173

Location

United States

Related Subject Headings

  • Spermatozoa
  • Rats, Sprague-Dawley
  • Rats
  • Nucleus Accumbens
  • Nerve Tissue Proteins
  • Marijuana Abuse
  • Male
  • Introns
  • Humans
  • Dronabinol