Comparison of seven methods for DNA extraction from prosomata of the acorn barnacle, Amphibalanus amphitrite.
Publication
, Journal Article
Schultzhaus, JN; Taitt, CR; Orihuela, B; Smerchansky, M; Schultzhaus, ZS; Rittschof, D; Wahl, KJ; Spillmann, CM
Published in: Analytical biochemistry
December 2019
Next generation sequencing (NGS) technologies can provide an understanding of the molecular processes involved in marine fouling by Amphibalanus spp. barnacles. Here, seven methods for extracting DNA from A. amphitrite prosomata were assessed with respect to recovery, purity and size distribution. Methods incorporating organic extractions generally resulted in low recovery of fragmented DNA. The most promising method was the commercial E.Z.N.A. Blood DNA Mini kit, which provided tens of micrograms of DNA of sufficient molecular weight for use in long-read NGS library preparation. Other kits resulted in DNA preps suitable for short read length NGS platforms.
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Published In
Analytical biochemistry
DOI
EISSN
1096-0309
ISSN
0003-2697
Publication Date
December 2019
Volume
586
Start / End Page
113441
Related Subject Headings
- Thoracica
- Organic Chemicals
- Molecular Weight
- High-Throughput Nucleotide Sequencing
- DNA
- Biochemistry & Molecular Biology
- Animals
- 3401 Analytical chemistry
- 3101 Biochemistry and cell biology
- 0601 Biochemistry and Cell Biology
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Schultzhaus, J. N., Taitt, C. R., Orihuela, B., Smerchansky, M., Schultzhaus, Z. S., Rittschof, D., … Spillmann, C. M. (2019). Comparison of seven methods for DNA extraction from prosomata of the acorn barnacle, Amphibalanus amphitrite. Analytical Biochemistry, 586, 113441. https://doi.org/10.1016/j.ab.2019.113441
Schultzhaus, Janna N., Chris R. Taitt, Beatriz Orihuela, Madeline Smerchansky, Zachary S. Schultzhaus, Daniel Rittschof, Kathryn J. Wahl, and Christopher M. Spillmann. “Comparison of seven methods for DNA extraction from prosomata of the acorn barnacle, Amphibalanus amphitrite.” Analytical Biochemistry 586 (December 2019): 113441. https://doi.org/10.1016/j.ab.2019.113441.
Schultzhaus JN, Taitt CR, Orihuela B, Smerchansky M, Schultzhaus ZS, Rittschof D, et al. Comparison of seven methods for DNA extraction from prosomata of the acorn barnacle, Amphibalanus amphitrite. Analytical biochemistry. 2019 Dec;586:113441.
Schultzhaus, Janna N., et al. “Comparison of seven methods for DNA extraction from prosomata of the acorn barnacle, Amphibalanus amphitrite.” Analytical Biochemistry, vol. 586, Dec. 2019, p. 113441. Epmc, doi:10.1016/j.ab.2019.113441.
Schultzhaus JN, Taitt CR, Orihuela B, Smerchansky M, Schultzhaus ZS, Rittschof D, Wahl KJ, Spillmann CM. Comparison of seven methods for DNA extraction from prosomata of the acorn barnacle, Amphibalanus amphitrite. Analytical biochemistry. 2019 Dec;586:113441.
Published In
Analytical biochemistry
DOI
EISSN
1096-0309
ISSN
0003-2697
Publication Date
December 2019
Volume
586
Start / End Page
113441
Related Subject Headings
- Thoracica
- Organic Chemicals
- Molecular Weight
- High-Throughput Nucleotide Sequencing
- DNA
- Biochemistry & Molecular Biology
- Animals
- 3401 Analytical chemistry
- 3101 Biochemistry and cell biology
- 0601 Biochemistry and Cell Biology