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Fine mapping of the Pc locus of Sorghum bicolor, a gene controlling the reaction to a fungal pathogen and its host-selective toxin.

Publication ,  Journal Article
Nagy, ED; Lee, T-C; Ramakrishna, W; Xu, Z; Klein, PE; SanMiguel, P; Cheng, C-P; Li, J; Devos, KM; Schertz, K; Dunkle, L; Bennetzen, JL
Published in: Theor Appl Genet
April 2007

Milo disease in sorghum is caused by isolates of the soil-borne fungus Periconia circinata that produce PC-toxin. Susceptibility to milo disease is conditioned by a single, semi-dominant gene, termed Pc. The susceptible allele (Pc) converts to a resistant form (pc) spontaneously at a gametic frequency of 10(-3) to 10(-4). A high-density genetic map was constructed around the Pc locus using DNA markers, allowing the Pc gene to be delimited to a 0.9 cM region on the short arm of sorghum chromosome 9. Physically, the Pc-region was covered by a single BAC clone. Sequence analysis of this BAC revealed twelve gene candidates. Several of the predicted genes in the region are homologous to disease resistance loci, including one NBS-LRR resistance gene analogue that is present in multiple tandem copies. Analysis of pc isolines derived from Pc/Pc sorghum suggests that one or more members of this NBS-LRR gene family are the Pc genes that condition susceptibility.

Duke Scholars

Published In

Theor Appl Genet

DOI

ISSN

0040-5752

Publication Date

April 2007

Volume

114

Issue

6

Start / End Page

961 / 970

Location

Germany

Related Subject Headings

  • Toxins, Biological
  • Sorghum
  • Sequence Homology, Amino Acid
  • Recombination, Genetic
  • Polymorphism, Single-Stranded Conformational
  • Plant Biology & Botany
  • Physical Chromosome Mapping
  • Molecular Sequence Data
  • Genotype
  • Genetic Markers
 

Citation

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Nagy, E. D., Lee, T.-C., Ramakrishna, W., Xu, Z., Klein, P. E., SanMiguel, P., … Bennetzen, J. L. (2007). Fine mapping of the Pc locus of Sorghum bicolor, a gene controlling the reaction to a fungal pathogen and its host-selective toxin. Theor Appl Genet, 114(6), 961–970. https://doi.org/10.1007/s00122-006-0481-1
Nagy, Ervin D., Tso-Ching Lee, Wusirika Ramakrishna, Zijun Xu, Patricia E. Klein, Phillip SanMiguel, Chiu-Ping Cheng, et al. “Fine mapping of the Pc locus of Sorghum bicolor, a gene controlling the reaction to a fungal pathogen and its host-selective toxin.Theor Appl Genet 114, no. 6 (April 2007): 961–70. https://doi.org/10.1007/s00122-006-0481-1.
Nagy ED, Lee T-C, Ramakrishna W, Xu Z, Klein PE, SanMiguel P, et al. Fine mapping of the Pc locus of Sorghum bicolor, a gene controlling the reaction to a fungal pathogen and its host-selective toxin. Theor Appl Genet. 2007 Apr;114(6):961–70.
Nagy, Ervin D., et al. “Fine mapping of the Pc locus of Sorghum bicolor, a gene controlling the reaction to a fungal pathogen and its host-selective toxin.Theor Appl Genet, vol. 114, no. 6, Apr. 2007, pp. 961–70. Pubmed, doi:10.1007/s00122-006-0481-1.
Nagy ED, Lee T-C, Ramakrishna W, Xu Z, Klein PE, SanMiguel P, Cheng C-P, Li J, Devos KM, Schertz K, Dunkle L, Bennetzen JL. Fine mapping of the Pc locus of Sorghum bicolor, a gene controlling the reaction to a fungal pathogen and its host-selective toxin. Theor Appl Genet. 2007 Apr;114(6):961–970.
Journal cover image

Published In

Theor Appl Genet

DOI

ISSN

0040-5752

Publication Date

April 2007

Volume

114

Issue

6

Start / End Page

961 / 970

Location

Germany

Related Subject Headings

  • Toxins, Biological
  • Sorghum
  • Sequence Homology, Amino Acid
  • Recombination, Genetic
  • Polymorphism, Single-Stranded Conformational
  • Plant Biology & Botany
  • Physical Chromosome Mapping
  • Molecular Sequence Data
  • Genotype
  • Genetic Markers