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International Ring Trial of a High Resolution Targeted Metabolomics and Lipidomics Platform for Serum and Plasma Analysis.

Publication ,  Journal Article
Thompson, JW; Adams, KJ; Adamski, J; Asad, Y; Borts, D; Bowden, JA; Byram, G; Dang, V; Dunn, WB; Fernandez, F; Fiehn, O; Gaul, DA; Hühmer, AF ...
Published in: Anal Chem
November 19, 2019

A challenge facing metabolomics in the analysis of large human cohorts is the cross-laboratory comparability of quantitative metabolomics measurements. In this study, 14 laboratories analyzed various blood specimens using a common experimental protocol provided with the Biocrates AbsoluteIDQ p400HR kit, to quantify up to 408 metabolites. The specimens included human plasma and serum from male and female donors, mouse and rat plasma, as well as NIST SRM 1950 reference plasma. The metabolite classes covered range from polar (e.g., amino acids and biogenic amines) to nonpolar (e.g., diacyl- and triacyl-glycerols), and they span 11 common metabolite classes. The manuscript describes a strict system suitability testing (SST) criteria used to evaluate each laboratory's readiness to perform the assay, and provides the SST Skyline documents for public dissemination. The study found approximately 250 metabolites were routinely quantified in the sample types tested, using Orbitrap instruments. Interlaboratory variance for the NIST SRM-1950 has a median of 10% for amino acids, 24% for biogenic amines, 38% for acylcarnitines, 25% for glycerolipids, 23% for glycerophospholipids, 16% for cholesteryl esters, 15% for sphingolipids, and 9% for hexoses. Comparing to consensus values for NIST SRM-1950, nearly 80% of comparable analytes demonstrated bias of <50% from the reference value. The findings of this study result in recommendations of best practices for system suitability, quality control, and calibration. We demonstrate that with appropriate controls, high-resolution metabolomics can provide accurate results with good precision across laboratories, and the p400HR therefore is a reliable approach for generating consistent and comparable metabolomics data.

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Published In

Anal Chem

DOI

EISSN

1520-6882

Publication Date

November 19, 2019

Volume

91

Issue

22

Start / End Page

14407 / 14416

Location

United States

Related Subject Headings

  • Reproducibility of Results
  • Rats
  • Mice
  • Metabolomics
  • Metabolome
  • Mass Spectrometry
  • Male
  • Lipids
  • Lipidomics
  • Limit of Detection
 

Citation

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Thompson, J. W., Adams, K. J., Adamski, J., Asad, Y., Borts, D., Bowden, J. A., … Moseley, M. A. (2019). International Ring Trial of a High Resolution Targeted Metabolomics and Lipidomics Platform for Serum and Plasma Analysis. Anal Chem, 91(22), 14407–14416. https://doi.org/10.1021/acs.analchem.9b02908
Thompson, J Will, Kendra J. Adams, Jerzy Adamski, Yasmin Asad, David Borts, John A. Bowden, Gregory Byram, et al. “International Ring Trial of a High Resolution Targeted Metabolomics and Lipidomics Platform for Serum and Plasma Analysis.Anal Chem 91, no. 22 (November 19, 2019): 14407–16. https://doi.org/10.1021/acs.analchem.9b02908.
Thompson JW, Adams KJ, Adamski J, Asad Y, Borts D, Bowden JA, et al. International Ring Trial of a High Resolution Targeted Metabolomics and Lipidomics Platform for Serum and Plasma Analysis. Anal Chem. 2019 Nov 19;91(22):14407–16.
Thompson, J. Will, et al. “International Ring Trial of a High Resolution Targeted Metabolomics and Lipidomics Platform for Serum and Plasma Analysis.Anal Chem, vol. 91, no. 22, Nov. 2019, pp. 14407–16. Pubmed, doi:10.1021/acs.analchem.9b02908.
Thompson JW, Adams KJ, Adamski J, Asad Y, Borts D, Bowden JA, Byram G, Dang V, Dunn WB, Fernandez F, Fiehn O, Gaul DA, Hühmer AF, Kalli A, Koal T, Koeniger S, Mandal R, Meier F, Naser FJ, O’Neil D, Pal A, Patti GJ, Pham-Tuan H, Prehn C, Raynaud FI, Shen T, Southam AD, St John-Williams L, Sulek K, Vasilopoulou CG, Viant M, Winder CL, Wishart D, Zhang L, Zheng J, Moseley MA. International Ring Trial of a High Resolution Targeted Metabolomics and Lipidomics Platform for Serum and Plasma Analysis. Anal Chem. 2019 Nov 19;91(22):14407–14416.
Journal cover image

Published In

Anal Chem

DOI

EISSN

1520-6882

Publication Date

November 19, 2019

Volume

91

Issue

22

Start / End Page

14407 / 14416

Location

United States

Related Subject Headings

  • Reproducibility of Results
  • Rats
  • Mice
  • Metabolomics
  • Metabolome
  • Mass Spectrometry
  • Male
  • Lipids
  • Lipidomics
  • Limit of Detection