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Acute Exposure to Permethrin Modulates Behavioral Functions, Redox, and Bioenergetics Parameters and Induces DNA Damage and Cell Death in Larval Zebrafish.

Publication ,  Journal Article
Nunes, MEM; Schimith, LE; da Costa-Silva, DG; Lopes, AR; Leandro, LP; Martins, IK; de Mello, RS; Hartmann, DD; de Carvalho, NR; da Rosa, PC ...
Published in: Oxidative medicine and cellular longevity
January 2019

Permethrin (PM) is a synthetic pyrethroid insecticide widely used as domestic repellent. Damage effects to nontarget organisms have been reported, particularly in the early stages of development. Studies indicate redox unbalance as secondary PM effect. Therefore, our goal was to investigate the acute PM effects on larval zebrafish. Larvae (6 days postfertilization) were exposed to PM (25-600 μg/L) during 24 hours, and 50% lethal concentration was estimated. For subsequent assays, the sublethal PM concentrations of 25 and 50 μg/L were used. PM increased anxiety-like behaviors according to the Novel Tank and Light-Dark tests. At the molecular level, PM induced increased ROS, which may be related to the increased lipid peroxidation, DNA damage, and apoptosis detected in PM-exposed organisms. In parallel, upregulation of the antioxidant system was detected after PM exposure, with increased superoxide dismutase, glutathione S-transferase and glutathione reductase activities, and thiol levels. The increased of Nrf2 target genes and the activation of an electrophile response element-driven reporter Tg(EPRE:LUC-EGFP) suggest that the Nrf2 pathway can mediate a fast response to PM, leading to antioxidant amplification. By using high-resolution respirometry, we found that exposure to PM decreased the oxygen consumption in all respiratory stages, disrupting the oxidative phosphorylation and inhibiting the electron transfer system, leading to decrease in bioenergetics capacity. In addition, PM led to increases of residual oxygen consumption and changes in substrate control ratio. Glucose metabolism seems to be affected by PM, with increased lactate dehydrogenase and decreased citrate synthase activities. Taken together, our results demonstrated the adverse effects of acute sublethal PM concentrations during larval development in zebrafish, causing apparent mitochondrial dysfunction, indicating a potential mechanism to redox unbalance and oxidative stress, which may be linked to the detected cell death and alterations in normal behavior patterns caused by acute PM exposure.

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Published In

Oxidative medicine and cellular longevity

DOI

EISSN

1942-0994

ISSN

1942-0900

Publication Date

January 2019

Volume

2019

Start / End Page

9149203

Related Subject Headings

  • Zebrafish
  • Reactive Oxygen Species
  • Permethrin
  • Oxidative Stress
  • Oxidation-Reduction
  • Mitochondria
  • Larva
  • Insecticides
  • Energy Metabolism
  • DNA Damage
 

Citation

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Nunes, M. E. M., Schimith, L. E., da Costa-Silva, D. G., Lopes, A. R., Leandro, L. P., Martins, I. K., … Franco, J. L. (2019). Acute Exposure to Permethrin Modulates Behavioral Functions, Redox, and Bioenergetics Parameters and Induces DNA Damage and Cell Death in Larval Zebrafish. Oxidative Medicine and Cellular Longevity, 2019, 9149203. https://doi.org/10.1155/2019/9149203
Nunes, Mauro Eugênio Medina, Lucia Emanueli Schimith, Dennis Guilherme da Costa-Silva, Andressa Rubim Lopes, Luana Paganotto Leandro, Illana Kemmerich Martins, Renata Siqueira de Mello, et al. “Acute Exposure to Permethrin Modulates Behavioral Functions, Redox, and Bioenergetics Parameters and Induces DNA Damage and Cell Death in Larval Zebrafish.Oxidative Medicine and Cellular Longevity 2019 (January 2019): 9149203. https://doi.org/10.1155/2019/9149203.
Nunes MEM, Schimith LE, da Costa-Silva DG, Lopes AR, Leandro LP, Martins IK, et al. Acute Exposure to Permethrin Modulates Behavioral Functions, Redox, and Bioenergetics Parameters and Induces DNA Damage and Cell Death in Larval Zebrafish. Oxidative medicine and cellular longevity. 2019 Jan;2019:9149203.
Nunes, Mauro Eugênio Medina, et al. “Acute Exposure to Permethrin Modulates Behavioral Functions, Redox, and Bioenergetics Parameters and Induces DNA Damage and Cell Death in Larval Zebrafish.Oxidative Medicine and Cellular Longevity, vol. 2019, Jan. 2019, p. 9149203. Epmc, doi:10.1155/2019/9149203.
Nunes MEM, Schimith LE, da Costa-Silva DG, Lopes AR, Leandro LP, Martins IK, de Mello RS, Hartmann DD, de Carvalho NR, da Rosa PC, Trevisan R, Di Giulio RT, Posser T, Franco JL. Acute Exposure to Permethrin Modulates Behavioral Functions, Redox, and Bioenergetics Parameters and Induces DNA Damage and Cell Death in Larval Zebrafish. Oxidative medicine and cellular longevity. 2019 Jan;2019:9149203.

Published In

Oxidative medicine and cellular longevity

DOI

EISSN

1942-0994

ISSN

1942-0900

Publication Date

January 2019

Volume

2019

Start / End Page

9149203

Related Subject Headings

  • Zebrafish
  • Reactive Oxygen Species
  • Permethrin
  • Oxidative Stress
  • Oxidation-Reduction
  • Mitochondria
  • Larva
  • Insecticides
  • Energy Metabolism
  • DNA Damage