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Dual role of the exocyst in AMPA receptor targeting and insertion into the postsynaptic membrane.

Publication ,  Journal Article
Gerges, NZ; Backos, DS; Rupasinghe, CN; Spaller, MR; Esteban, JA
Published in: The EMBO journal
April 2006

Intracellular membrane trafficking of glutamate receptors at excitatory synapses is critical for synaptic function. However, little is known about the specialized trafficking events occurring at the postsynaptic membrane. We have found that two components of the exocyst complex, Sec8 and Exo70, separately control synaptic targeting and insertion of AMPA-type glutamate receptors. Sec8 controls the directional movement of receptors towards synapses through PDZ-dependent interactions. In contrast, Exo70 mediates receptor insertion at the postsynaptic membrane, but it does not participate in receptor targeting. Thus, interference with Exo70 function accumulates AMPA receptors inside the spine, forming a complex physically associated, but not yet fused with the postsynaptic membrane. Electron microscopic analysis of these complexes indicates that Exo70 mediates AMPA receptor insertion directly within the postsynaptic density, rather than at extrasynaptic membranes. Therefore, we propose a molecular and anatomical model that dissects AMPA receptor sorting and synaptic delivery within the spine, and uncovers new functions of the exocyst at the postsynaptic membrane.

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Published In

The EMBO journal

DOI

EISSN

1460-2075

ISSN

0261-4189

Publication Date

April 2006

Volume

25

Issue

8

Start / End Page

1623 / 1634

Related Subject Headings

  • Vesicular Transport Proteins
  • Synaptosomes
  • Synaptic Transmission
  • Synaptic Membranes
  • Synapses
  • Receptors, AMPA
  • Rats
  • Protein Transport
  • Patch-Clamp Techniques
  • Organ Culture Techniques
 

Citation

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ICMJE
MLA
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Gerges, N. Z., Backos, D. S., Rupasinghe, C. N., Spaller, M. R., & Esteban, J. A. (2006). Dual role of the exocyst in AMPA receptor targeting and insertion into the postsynaptic membrane. The EMBO Journal, 25(8), 1623–1634. https://doi.org/10.1038/sj.emboj.7601065
Gerges, Nashaat Z., Donald S. Backos, Chamila N. Rupasinghe, Mark R. Spaller, and José A. Esteban. “Dual role of the exocyst in AMPA receptor targeting and insertion into the postsynaptic membrane.The EMBO Journal 25, no. 8 (April 2006): 1623–34. https://doi.org/10.1038/sj.emboj.7601065.
Gerges NZ, Backos DS, Rupasinghe CN, Spaller MR, Esteban JA. Dual role of the exocyst in AMPA receptor targeting and insertion into the postsynaptic membrane. The EMBO journal. 2006 Apr;25(8):1623–34.
Gerges, Nashaat Z., et al. “Dual role of the exocyst in AMPA receptor targeting and insertion into the postsynaptic membrane.The EMBO Journal, vol. 25, no. 8, Apr. 2006, pp. 1623–34. Epmc, doi:10.1038/sj.emboj.7601065.
Gerges NZ, Backos DS, Rupasinghe CN, Spaller MR, Esteban JA. Dual role of the exocyst in AMPA receptor targeting and insertion into the postsynaptic membrane. The EMBO journal. 2006 Apr;25(8):1623–1634.

Published In

The EMBO journal

DOI

EISSN

1460-2075

ISSN

0261-4189

Publication Date

April 2006

Volume

25

Issue

8

Start / End Page

1623 / 1634

Related Subject Headings

  • Vesicular Transport Proteins
  • Synaptosomes
  • Synaptic Transmission
  • Synaptic Membranes
  • Synapses
  • Receptors, AMPA
  • Rats
  • Protein Transport
  • Patch-Clamp Techniques
  • Organ Culture Techniques