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Nuclear Localized O-Fucosyltransferase SPY Facilitates PRR5 Proteolysis to Fine-Tune the Pace of Arabidopsis Circadian Clock.

Publication ,  Journal Article
Wang, Y; He, Y; Su, C; Zentella, R; Sun, T-P; Wang, L
Published in: Molecular plant
March 2020

Post-translational modifications play essential roles in finely modulating eukaryotic circadian clock systems. In plants, the effects of O-glycosylation on the circadian clock and the underlying mechanisms remain largely unknown. The O-fucosyltransferase SPINDLY (SPY) and the O-GlcNAc transferase SECRET AGENT (SEC) are two prominent O-glycosylation enzymes in higher plants, with both overlapped and unique functions in plant growth and development. Unlike the critical role of O-GlcNAc in regulating the animal circadian clock, here we report that nuclear-localized SPY, but not SEC, specifically modulates the pace of the Arabidopsis circadian clock. By identifying the interactome of SPY, we identified PSEUDO-RESPONSE REGULATOR 5 (PRR5), one of the core circadian clock components, as a new SPY-interacting protein. PRR5 can be O-fucosylated by SPY in planta, while point mutation in the catalytic domain of SPY abolishes the O-fucosylation of PRR5. The protein abundance of PRR5 is strongly increased in spy mutants, while the degradation rate of PRR5 is much reduced, suggesting that PRR5 proteolysis is promoted by SPY-mediated O-fucosylation. Moreover, multiple lines of genetic evidence indicate that PRR5 is a major downstream target of SPY to specifically mediate its modulation of the circadian clock. Collectively, our findings provide novel insights into the specific role of the O-fucosyltransferase activity of SPY in modulating the circadian clock and implicate that O-glycosylation might play an evolutionarily conserved role in modulating the circadian clock system, via O-GlcNAcylation in mammals, but via O-fucosylation in higher plants.

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Published In

Molecular plant

DOI

EISSN

1752-9867

ISSN

1674-2052

Publication Date

March 2020

Volume

13

Issue

3

Start / End Page

446 / 458

Related Subject Headings

  • Transcription Factors
  • Repressor Proteins
  • Proteolysis
  • Plant Biology & Botany
  • Glycosylation
  • Circadian Clocks
  • Cell Nucleus
  • Arabidopsis Proteins
  • Arabidopsis
  • Active Transport, Cell Nucleus
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Wang, Y., He, Y., Su, C., Zentella, R., Sun, T.-P., & Wang, L. (2020). Nuclear Localized O-Fucosyltransferase SPY Facilitates PRR5 Proteolysis to Fine-Tune the Pace of Arabidopsis Circadian Clock. Molecular Plant, 13(3), 446–458. https://doi.org/10.1016/j.molp.2019.12.013
Wang, Yan, Yuqing He, Chen Su, Rodolfo Zentella, Tai-Ping Sun, and Lei Wang. “Nuclear Localized O-Fucosyltransferase SPY Facilitates PRR5 Proteolysis to Fine-Tune the Pace of Arabidopsis Circadian Clock.Molecular Plant 13, no. 3 (March 2020): 446–58. https://doi.org/10.1016/j.molp.2019.12.013.
Wang Y, He Y, Su C, Zentella R, Sun T-P, Wang L. Nuclear Localized O-Fucosyltransferase SPY Facilitates PRR5 Proteolysis to Fine-Tune the Pace of Arabidopsis Circadian Clock. Molecular plant. 2020 Mar;13(3):446–58.
Wang, Yan, et al. “Nuclear Localized O-Fucosyltransferase SPY Facilitates PRR5 Proteolysis to Fine-Tune the Pace of Arabidopsis Circadian Clock.Molecular Plant, vol. 13, no. 3, Mar. 2020, pp. 446–58. Epmc, doi:10.1016/j.molp.2019.12.013.
Wang Y, He Y, Su C, Zentella R, Sun T-P, Wang L. Nuclear Localized O-Fucosyltransferase SPY Facilitates PRR5 Proteolysis to Fine-Tune the Pace of Arabidopsis Circadian Clock. Molecular plant. 2020 Mar;13(3):446–458.
Journal cover image

Published In

Molecular plant

DOI

EISSN

1752-9867

ISSN

1674-2052

Publication Date

March 2020

Volume

13

Issue

3

Start / End Page

446 / 458

Related Subject Headings

  • Transcription Factors
  • Repressor Proteins
  • Proteolysis
  • Plant Biology & Botany
  • Glycosylation
  • Circadian Clocks
  • Cell Nucleus
  • Arabidopsis Proteins
  • Arabidopsis
  • Active Transport, Cell Nucleus