Scope and Predictive Genetic/Phenotypic Signatures of Bicarbonate (NaHCO3) Responsiveness and β-Lactam Sensitization in Methicillin-Resistant Staphylococcus aureus.
Publication
, Journal Article
Ersoy, SC; Otmishi, M; Milan, VT; Li, L; Pak, Y; Mediavilla, J; Chen, L; Kreiswirth, B; Chambers, HF; Proctor, RA; Xiong, YQ; Fowler, VG; Bayer, AS
Published in: Antimicrob Agents Chemother
April 21, 2020
Addition of sodium bicarbonate (NaHCO3) to standard antimicrobial susceptibility testing medium reveals certain methicillin-resistant Staphylococcus aureus (MRSA) strains to be highly susceptible to β-lactams. We investigated the prevalence of this phenotype (NaHCO3 responsiveness) to two β-lactams among 58 clinical MRSA bloodstream isolates. Of note, ∼75% and ∼36% of isolates displayed the NaHCO3 responsiveness phenotype to cefazolin (CFZ) and oxacillin (OXA), respectively. Neither intrinsic β-lactam MICs in standard Mueller-Hinton broth (MHB) nor population analysis profiles were predictive of this phenotype. Several genotypic markers (clonal complex 8 [CC8]; agr I and spa t008) were associated with NaHCO3 responsiveness for OXA.
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Published In
Antimicrob Agents Chemother
DOI
EISSN
1098-6596
Publication Date
April 21, 2020
Volume
64
Issue
5
Location
United States
Related Subject Headings
- beta-Lactams
- Staphylococcal Infections
- Sodium Bicarbonate
- Prevalence
- Predictive Value of Tests
- Phenotype
- Oxacillin
- Microbiology
- Microbial Sensitivity Tests
- Methicillin-Resistant Staphylococcus aureus
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MLA
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Ersoy, S. C., Otmishi, M., Milan, V. T., Li, L., Pak, Y., Mediavilla, J., … Bayer, A. S. (2020). Scope and Predictive Genetic/Phenotypic Signatures of Bicarbonate (NaHCO3) Responsiveness and β-Lactam Sensitization in Methicillin-Resistant Staphylococcus aureus. Antimicrob Agents Chemother, 64(5). https://doi.org/10.1128/AAC.02445-19
Ersoy, Selvi C., Mariam Otmishi, Vanessa T. Milan, Liang Li, Youngju Pak, Jose Mediavilla, Liang Chen, et al. “Scope and Predictive Genetic/Phenotypic Signatures of Bicarbonate (NaHCO3) Responsiveness and β-Lactam Sensitization in Methicillin-Resistant Staphylococcus aureus.” Antimicrob Agents Chemother 64, no. 5 (April 21, 2020). https://doi.org/10.1128/AAC.02445-19.
Ersoy SC, Otmishi M, Milan VT, Li L, Pak Y, Mediavilla J, et al. Scope and Predictive Genetic/Phenotypic Signatures of Bicarbonate (NaHCO3) Responsiveness and β-Lactam Sensitization in Methicillin-Resistant Staphylococcus aureus. Antimicrob Agents Chemother. 2020 Apr 21;64(5).
Ersoy, Selvi C., et al. “Scope and Predictive Genetic/Phenotypic Signatures of Bicarbonate (NaHCO3) Responsiveness and β-Lactam Sensitization in Methicillin-Resistant Staphylococcus aureus.” Antimicrob Agents Chemother, vol. 64, no. 5, Apr. 2020. Pubmed, doi:10.1128/AAC.02445-19.
Ersoy SC, Otmishi M, Milan VT, Li L, Pak Y, Mediavilla J, Chen L, Kreiswirth B, Chambers HF, Proctor RA, Xiong YQ, Fowler VG, Bayer AS. Scope and Predictive Genetic/Phenotypic Signatures of Bicarbonate (NaHCO3) Responsiveness and β-Lactam Sensitization in Methicillin-Resistant Staphylococcus aureus. Antimicrob Agents Chemother. 2020 Apr 21;64(5).
Published In
Antimicrob Agents Chemother
DOI
EISSN
1098-6596
Publication Date
April 21, 2020
Volume
64
Issue
5
Location
United States
Related Subject Headings
- beta-Lactams
- Staphylococcal Infections
- Sodium Bicarbonate
- Prevalence
- Predictive Value of Tests
- Phenotype
- Oxacillin
- Microbiology
- Microbial Sensitivity Tests
- Methicillin-Resistant Staphylococcus aureus