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A Robust Bioderived Wavelength-Specific Photosensor Based on BLUF Proteins.

Publication ,  Journal Article
Tong, J; Zhang, P; Zhang, L; Zhang, D; Beratan, DN; Song, H; Wang, Y; Li, T
Published in: Sensors and actuators. B, Chemical
May 2020

Photosensitive proteins are naturally evolved photosensors that often respond to light signals of specific wavelengths. However, their poor stability under ambient conditions hinders their applications in non-biological settings. In this proof-of-principle study, we grafted the blue light using flavin (BLUF) protein reconstructed with flavin adenine dinucleotide (FAD) or roseoflavin (RoF) onto pristine graphene, and achieved selective sensitivity at 450 nm or 500 nm, respectively. We improved the thermal and operational stability substantially via structure-guided cross-linking, achieving 6-month stability under ambient condition and normal operation at temperatures up to 200 °C. Furthermore, the device exhibited rare negative photoconductivity behavior. The origins of this negative photoconductivity behavior were elucidated via a combination of experimental and theoretical analysis. In the photoelectric conversion studies, holes from photoexcited flavin migrated to graphene and recombined with electrons. The device allows facile modulation and detection of charge transfer, and provides a versatile platform for future studies of photoinduced charge transfer in biosensors as well as the development of stable wavelength-selective biophotosensors.

Duke Scholars

Published In

Sensors and actuators. B, Chemical

DOI

ISSN

0925-4005

Publication Date

May 2020

Volume

310

Start / End Page

127838

Related Subject Headings

  • Analytical Chemistry
  • 4016 Materials engineering
  • 4004 Chemical engineering
  • 3401 Analytical chemistry
  • 0912 Materials Engineering
  • 0301 Analytical Chemistry
  • 0205 Optical Physics
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Tong, J., Zhang, P., Zhang, L., Zhang, D., Beratan, D. N., Song, H., … Li, T. (2020). A Robust Bioderived Wavelength-Specific Photosensor Based on BLUF Proteins. Sensors and Actuators. B, Chemical, 310, 127838. https://doi.org/10.1016/j.snb.2020.127838
Tong, Jing, Peng Zhang, Lei Zhang, Dongwei Zhang, David N. Beratan, Haifeng Song, Yi Wang, and Tie Li. “A Robust Bioderived Wavelength-Specific Photosensor Based on BLUF Proteins.Sensors and Actuators. B, Chemical 310 (May 2020): 127838. https://doi.org/10.1016/j.snb.2020.127838.
Tong J, Zhang P, Zhang L, Zhang D, Beratan DN, Song H, et al. A Robust Bioderived Wavelength-Specific Photosensor Based on BLUF Proteins. Sensors and actuators B, Chemical. 2020 May;310:127838.
Tong, Jing, et al. “A Robust Bioderived Wavelength-Specific Photosensor Based on BLUF Proteins.Sensors and Actuators. B, Chemical, vol. 310, May 2020, p. 127838. Epmc, doi:10.1016/j.snb.2020.127838.
Tong J, Zhang P, Zhang L, Zhang D, Beratan DN, Song H, Wang Y, Li T. A Robust Bioderived Wavelength-Specific Photosensor Based on BLUF Proteins. Sensors and actuators B, Chemical. 2020 May;310:127838.
Journal cover image

Published In

Sensors and actuators. B, Chemical

DOI

ISSN

0925-4005

Publication Date

May 2020

Volume

310

Start / End Page

127838

Related Subject Headings

  • Analytical Chemistry
  • 4016 Materials engineering
  • 4004 Chemical engineering
  • 3401 Analytical chemistry
  • 0912 Materials Engineering
  • 0301 Analytical Chemistry
  • 0205 Optical Physics