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Structures of AAA protein translocase Bcs1 suggest translocation mechanism of a folded protein.

Publication ,  Journal Article
Tang, WK; Borgnia, MJ; Hsu, AL; Esser, L; Fox, T; de Val, N; Xia, D
Published in: Nat Struct Mol Biol
February 2020

The mitochondrial membrane-bound AAA protein Bcs1 translocate substrates across the mitochondrial inner membrane without previous unfolding. One substrate of Bcs1 is the iron-sulfur protein (ISP), a subunit of the respiratory Complex III. How Bcs1 translocates ISP across the membrane is unknown. Here we report structures of mouse Bcs1 in two different conformations, representing three nucleotide states. The apo and ADP-bound structures reveal a homo-heptamer and show a large putative substrate-binding cavity accessible to the matrix space. ATP binding drives a contraction of the cavity by concerted motion of the ATPase domains, which could push substrate across the membrane. Our findings shed light on the potential mechanism of translocating folded proteins across a membrane, offer insights into the assembly process of Complex III and allow mapping of human disease-associated mutations onto the Bcs1 structure.

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Published In

Nat Struct Mol Biol

DOI

EISSN

1545-9985

Publication Date

February 2020

Volume

27

Issue

2

Start / End Page

202 / 209

Location

United States

Related Subject Headings

  • Protein Transport
  • Protein Multimerization
  • Protein Folding
  • Protein Domains
  • Protein Conformation
  • Molecular Chaperones
  • Models, Molecular
  • Mitochondrial Proteins
  • Mice
  • Developmental Biology
 

Citation

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Tang, W. K., Borgnia, M. J., Hsu, A. L., Esser, L., Fox, T., de Val, N., & Xia, D. (2020). Structures of AAA protein translocase Bcs1 suggest translocation mechanism of a folded protein. Nat Struct Mol Biol, 27(2), 202–209. https://doi.org/10.1038/s41594-020-0373-0
Tang, Wai Kwan, Mario J. Borgnia, Allen L. Hsu, Lothar Esser, Tara Fox, Natalia de Val, and Di Xia. “Structures of AAA protein translocase Bcs1 suggest translocation mechanism of a folded protein.Nat Struct Mol Biol 27, no. 2 (February 2020): 202–9. https://doi.org/10.1038/s41594-020-0373-0.
Tang WK, Borgnia MJ, Hsu AL, Esser L, Fox T, de Val N, et al. Structures of AAA protein translocase Bcs1 suggest translocation mechanism of a folded protein. Nat Struct Mol Biol. 2020 Feb;27(2):202–9.
Tang, Wai Kwan, et al. “Structures of AAA protein translocase Bcs1 suggest translocation mechanism of a folded protein.Nat Struct Mol Biol, vol. 27, no. 2, Feb. 2020, pp. 202–09. Pubmed, doi:10.1038/s41594-020-0373-0.
Tang WK, Borgnia MJ, Hsu AL, Esser L, Fox T, de Val N, Xia D. Structures of AAA protein translocase Bcs1 suggest translocation mechanism of a folded protein. Nat Struct Mol Biol. 2020 Feb;27(2):202–209.

Published In

Nat Struct Mol Biol

DOI

EISSN

1545-9985

Publication Date

February 2020

Volume

27

Issue

2

Start / End Page

202 / 209

Location

United States

Related Subject Headings

  • Protein Transport
  • Protein Multimerization
  • Protein Folding
  • Protein Domains
  • Protein Conformation
  • Molecular Chaperones
  • Models, Molecular
  • Mitochondrial Proteins
  • Mice
  • Developmental Biology