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Roles of cytoplasmic Ca2+ and intracellular Ca2+ stores in induction and suppression of apoptosis in S49 cells.

Publication ,  Journal Article
Bian, X; Hughes, FM; Huang, Y; Cidlowski, JA; Putney, JW
Published in: Am J Physiol
April 1997

The Ca2+-ATPase inhibitors, thapsigargin and cyclopiazonic acid, depleted intracellular Ca2+ stores, induced large increases in intracellular Ca2+ concentration, and caused apoptosis in S49 cells. Removal of extracellular Ca2+ augmented apoptosis due to thapsigargin, indicating that depletion of Ca2+ from intracellular stores is responsible for apoptosis with this agent. Overexpression of the apoptosis suppressor, Bcl-2, inhibited apoptosis due to thapsigargin but did not affect thapsigargin-induced Ca2+ signaling. Dexamethasone induced apoptosis, diminished the size of the endoplasmic reticulum Ca2+ pool, and caused a small elevation of intracellular Ca2+. However, this elevation was not due to Ca2+ influx because the increase was similar in the presence or absence of Ca2+ in the medium. Furthermore, in contrast to the results with thapsigargin, apoptosis due to dexamethasone was unchanged in a Ca2+-free medium. These results indicate that depletion of Ca2+ stores initiates a pathway leading to apoptosis. Elevations in cytoplasmic Ca2+ appears to play a lesser role than previously thought in the actions of Bcl-2 and glucocorticoids.

Duke Scholars

Published In

Am J Physiol

DOI

ISSN

0002-9513

Publication Date

April 1997

Volume

272

Issue

4 Pt 1

Start / End Page

C1241 / C1249

Location

United States

Related Subject Headings

  • Thapsigargin
  • Signal Transduction
  • Proto-Oncogene Proteins c-bcl-2
  • Intracellular Membranes
  • Indoles
  • Glucocorticoids
  • Enzyme Inhibitors
  • Dexamethasone
  • Cytoplasm
  • Cell Line, Transformed
 

Citation

APA
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MLA
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Bian, X., Hughes, F. M., Huang, Y., Cidlowski, J. A., & Putney, J. W. (1997). Roles of cytoplasmic Ca2+ and intracellular Ca2+ stores in induction and suppression of apoptosis in S49 cells. Am J Physiol, 272(4 Pt 1), C1241–C1249. https://doi.org/10.1152/ajpcell.1997.272.4.C1241
Bian, X., F. M. Hughes, Y. Huang, J. A. Cidlowski, and J. W. Putney. “Roles of cytoplasmic Ca2+ and intracellular Ca2+ stores in induction and suppression of apoptosis in S49 cells.Am J Physiol 272, no. 4 Pt 1 (April 1997): C1241–49. https://doi.org/10.1152/ajpcell.1997.272.4.C1241.
Bian X, Hughes FM, Huang Y, Cidlowski JA, Putney JW. Roles of cytoplasmic Ca2+ and intracellular Ca2+ stores in induction and suppression of apoptosis in S49 cells. Am J Physiol. 1997 Apr;272(4 Pt 1):C1241–9.
Bian, X., et al. “Roles of cytoplasmic Ca2+ and intracellular Ca2+ stores in induction and suppression of apoptosis in S49 cells.Am J Physiol, vol. 272, no. 4 Pt 1, Apr. 1997, pp. C1241–49. Pubmed, doi:10.1152/ajpcell.1997.272.4.C1241.
Bian X, Hughes FM, Huang Y, Cidlowski JA, Putney JW. Roles of cytoplasmic Ca2+ and intracellular Ca2+ stores in induction and suppression of apoptosis in S49 cells. Am J Physiol. 1997 Apr;272(4 Pt 1):C1241–C1249.

Published In

Am J Physiol

DOI

ISSN

0002-9513

Publication Date

April 1997

Volume

272

Issue

4 Pt 1

Start / End Page

C1241 / C1249

Location

United States

Related Subject Headings

  • Thapsigargin
  • Signal Transduction
  • Proto-Oncogene Proteins c-bcl-2
  • Intracellular Membranes
  • Indoles
  • Glucocorticoids
  • Enzyme Inhibitors
  • Dexamethasone
  • Cytoplasm
  • Cell Line, Transformed