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Inhibition of neuroinflammation in BV2 microglia by the biflavonoid kolaviron is dependent on the Nrf2/ARE antioxidant protective mechanism.

Publication ,  Journal Article
Onasanwo, SA; Velagapudi, R; El-Bakoush, A; Olajide, OA
Published in: Mol Cell Biochem
March 2016

Kolaviron is a mixture of biflavonoids found in the nut of the West African edible seed Garcinia kola, and it has been reported to exhibit a wide range of pharmacological activities. In this study, we investigated the effects of kolaviron in neuroinflammation. The effects of kolaviron on the expression of nitric oxide/inducible nitric oxide synthase (iNOS), prostaglandin E2 (PGE2)/cyclooxygenase-2, cellular reactive oxygen species (ROS) and the pro-inflammatory cytokines were examined in lipopolysaccharide (LPS)-stimulated BV2 microglial cells. Molecular mechanisms of the effects of kolaviron on NF-κB and Nrf2/ARE signalling pathways were analysed by immunoblotting, binding assays and reporter assays. RNA interference was used to investigate the role of Nrf2 in the anti-inflammatory effect of kolaviron. Neuroprotective effect of kolaviron was assessed in a BV2 microglia/HT22 hippocampal neuron co-culture. Kolaviron inhibited the protein levels of NO/iNOS, PGE2/COX-2, cellular ROS and the pro-inflammatory cytokines (TNFα and IL-6) in LPS-stimulated microglia. Further mechanistic studies showed that kolaviron inhibited neuroinflammation by inhibiting IκB/NF-κB signalling pathway in LPS-activated BV2 microglia. Kolaviron produced antioxidant effect in BV2 microglia by increasing HO-1 via the Nrf2/antioxidant response element pathway. RNAi experiments revealed that Nrf2 is needed for the anti-inflammatory effects of kolaviron. Kolaviron protected HT22 neurons from neuroinflammation-induced toxicity. Kolaviron inhibits neuroinflammation through Nrf2-dependent mechanisms. This compound may therefore be beneficial in neuroinflammation-related neurodegenerative disorders.

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Published In

Mol Cell Biochem

DOI

EISSN

1573-4919

Publication Date

March 2016

Volume

414

Issue

1-2

Start / End Page

23 / 36

Location

Netherlands

Related Subject Headings

  • Tumor Necrosis Factor-alpha
  • Signal Transduction
  • Reactive Oxygen Species
  • Nitric Oxide Synthase Type II
  • Nitric Oxide
  • NF-kappa B
  • NF-E2-Related Factor 2
  • Microglia
  • Mice
  • Lipopolysaccharides
 

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Onasanwo, S. A., Velagapudi, R., El-Bakoush, A., & Olajide, O. A. (2016). Inhibition of neuroinflammation in BV2 microglia by the biflavonoid kolaviron is dependent on the Nrf2/ARE antioxidant protective mechanism. Mol Cell Biochem, 414(1–2), 23–36. https://doi.org/10.1007/s11010-016-2655-8
Onasanwo, Samuel A., Ravikanth Velagapudi, Abdelmeneim El-Bakoush, and Olumayokun A. Olajide. “Inhibition of neuroinflammation in BV2 microglia by the biflavonoid kolaviron is dependent on the Nrf2/ARE antioxidant protective mechanism.Mol Cell Biochem 414, no. 1–2 (March 2016): 23–36. https://doi.org/10.1007/s11010-016-2655-8.
Onasanwo SA, Velagapudi R, El-Bakoush A, Olajide OA. Inhibition of neuroinflammation in BV2 microglia by the biflavonoid kolaviron is dependent on the Nrf2/ARE antioxidant protective mechanism. Mol Cell Biochem. 2016 Mar;414(1–2):23–36.
Onasanwo, Samuel A., et al. “Inhibition of neuroinflammation in BV2 microglia by the biflavonoid kolaviron is dependent on the Nrf2/ARE antioxidant protective mechanism.Mol Cell Biochem, vol. 414, no. 1–2, Mar. 2016, pp. 23–36. Pubmed, doi:10.1007/s11010-016-2655-8.
Onasanwo SA, Velagapudi R, El-Bakoush A, Olajide OA. Inhibition of neuroinflammation in BV2 microglia by the biflavonoid kolaviron is dependent on the Nrf2/ARE antioxidant protective mechanism. Mol Cell Biochem. 2016 Mar;414(1–2):23–36.
Journal cover image

Published In

Mol Cell Biochem

DOI

EISSN

1573-4919

Publication Date

March 2016

Volume

414

Issue

1-2

Start / End Page

23 / 36

Location

Netherlands

Related Subject Headings

  • Tumor Necrosis Factor-alpha
  • Signal Transduction
  • Reactive Oxygen Species
  • Nitric Oxide Synthase Type II
  • Nitric Oxide
  • NF-kappa B
  • NF-E2-Related Factor 2
  • Microglia
  • Mice
  • Lipopolysaccharides