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Natural Selection Shapes Variation in Genome-wide Recombination Rate in Drosophila pseudoobscura.

Publication ,  Journal Article
Samuk, K; Manzano-Winkler, B; Ritz, KR; Noor, MAF
Published in: Current biology : CB
April 2020

While recombination is widely recognized to be a key modulator of numerous evolutionary phenomena, we have a poor understanding of how recombination rate itself varies and evolves within a species. Here, we performed a comprehensive study of recombination rate (rate of meiotic crossing over) in two natural populations of Drosophila pseudoobscura from Utah and Arizona, USA. We used an amplicon sequencing approach to obtain high-quality genotypes in approximately 8,000 individual backcrossed offspring (17 mapping populations with roughly 530 individuals each), for which we then quantified crossovers. Interestingly, variation in recombination rate within and between populations largely manifested as differences in genome-wide recombination rate rather than remodeling of the local recombination landscape. Comparing populations, we discovered individuals from the Utah population displayed on average 8% higher crossover rates than the Arizona population, a statistically significant difference. Using a QST-FST analysis, we found that this difference in crossover rate was dramatically higher than expected under neutrality, indicating that this difference may have been driven by natural selection. Finally, using a combination of short- and long-read whole-genome sequencing, we found no significant association between crossover rate and structural variation at the 200-400 kb scale. Our results demonstrate that (1) there is abundant variation in genome-wide crossover rate in natural populations, (2) at the 200-400 kb scale, recombination rate appears to vary largely genome-wide, rather than in specific intervals, and (3) interpopulation differences in recombination rate may be the result of local adaptation.

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Published In

Current biology : CB

DOI

EISSN

1879-0445

ISSN

0960-9822

Publication Date

April 2020

Volume

30

Issue

8

Start / End Page

1517 / 1528.e6

Related Subject Headings

  • Utah
  • Selection, Genetic
  • Male
  • Genome, Insect
  • Female
  • Drosophila
  • Developmental Biology
  • Crossing Over, Genetic
  • Arizona
  • Animals
 

Citation

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Samuk, K., Manzano-Winkler, B., Ritz, K. R., & Noor, M. A. F. (2020). Natural Selection Shapes Variation in Genome-wide Recombination Rate in Drosophila pseudoobscura. Current Biology : CB, 30(8), 1517-1528.e6. https://doi.org/10.1016/j.cub.2020.03.053
Samuk, Kieran, Brenda Manzano-Winkler, Kathryn R. Ritz, and Mohamed A. F. Noor. “Natural Selection Shapes Variation in Genome-wide Recombination Rate in Drosophila pseudoobscura.Current Biology : CB 30, no. 8 (April 2020): 1517-1528.e6. https://doi.org/10.1016/j.cub.2020.03.053.
Samuk K, Manzano-Winkler B, Ritz KR, Noor MAF. Natural Selection Shapes Variation in Genome-wide Recombination Rate in Drosophila pseudoobscura. Current biology : CB. 2020 Apr;30(8):1517-1528.e6.
Samuk, Kieran, et al. “Natural Selection Shapes Variation in Genome-wide Recombination Rate in Drosophila pseudoobscura.Current Biology : CB, vol. 30, no. 8, Apr. 2020, pp. 1517-1528.e6. Epmc, doi:10.1016/j.cub.2020.03.053.
Samuk K, Manzano-Winkler B, Ritz KR, Noor MAF. Natural Selection Shapes Variation in Genome-wide Recombination Rate in Drosophila pseudoobscura. Current biology : CB. 2020 Apr;30(8):1517-1528.e6.
Journal cover image

Published In

Current biology : CB

DOI

EISSN

1879-0445

ISSN

0960-9822

Publication Date

April 2020

Volume

30

Issue

8

Start / End Page

1517 / 1528.e6

Related Subject Headings

  • Utah
  • Selection, Genetic
  • Male
  • Genome, Insect
  • Female
  • Drosophila
  • Developmental Biology
  • Crossing Over, Genetic
  • Arizona
  • Animals