Skip to main content

Combined Atomic Force Microscope and Volumetric Light Sheet System for Correlative Force and Fluorescence Mechanobiology Studies.

Publication ,  Journal Article
Nelsen, E; Hobson, CM; Kern, ME; Hsiao, JP; O'Brien Iii, ET; Watanabe, T; Condon, BM; Boyce, M; Grinstein, S; Hahn, KM; Falvo, MR; Superfine, R
Published in: Sci Rep
May 18, 2020

The central goals of mechanobiology are to understand how cells generate force and how they respond to environmental mechanical stimuli. A full picture of these processes requires high-resolution, volumetric imaging with time-correlated force measurements. Here we present an instrument that combines an open-top, single-objective light sheet fluorescence microscope with an atomic force microscope (AFM), providing simultaneous volumetric imaging with high spatiotemporal resolution and high dynamic range force capability (10 pN - 100 nN). With this system we have captured lysosome trafficking, vimentin nuclear caging, and actin dynamics on the order of one second per single-cell volume. To showcase the unique advantages of combining Line Bessel light sheet imaging with AFM, we measured the forces exerted by a macrophage during FcɣR-mediated phagocytosis while performing both sequential two-color, fixed plane and volumetric imaging of F-actin. This unique instrument allows for a myriad of novel studies investigating the coupling of cellular dynamics and mechanical forces.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Sci Rep

DOI

EISSN

2045-2322

Publication Date

May 18, 2020

Volume

10

Issue

1

Start / End Page

8133

Location

England

Related Subject Headings

  • RAW 264.7 Cells
  • Phagocytosis
  • Microscopy, Fluorescence
  • Microscopy, Atomic Force
  • Mice
  • Macrophages
  • Humans
  • Hela Cells
  • HeLa Cells
  • Fluorescence
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Nelsen, E., Hobson, C. M., Kern, M. E., Hsiao, J. P., O’Brien Iii, E. T., Watanabe, T., … Superfine, R. (2020). Combined Atomic Force Microscope and Volumetric Light Sheet System for Correlative Force and Fluorescence Mechanobiology Studies. Sci Rep, 10(1), 8133. https://doi.org/10.1038/s41598-020-65205-8
Nelsen, E., C. M. Hobson, M. E. Kern, J. P. Hsiao, E. T. O’Brien Iii, T. Watanabe, B. M. Condon, et al. “Combined Atomic Force Microscope and Volumetric Light Sheet System for Correlative Force and Fluorescence Mechanobiology Studies.Sci Rep 10, no. 1 (May 18, 2020): 8133. https://doi.org/10.1038/s41598-020-65205-8.
Nelsen E, Hobson CM, Kern ME, Hsiao JP, O’Brien Iii ET, Watanabe T, et al. Combined Atomic Force Microscope and Volumetric Light Sheet System for Correlative Force and Fluorescence Mechanobiology Studies. Sci Rep. 2020 May 18;10(1):8133.
Nelsen, E., et al. “Combined Atomic Force Microscope and Volumetric Light Sheet System for Correlative Force and Fluorescence Mechanobiology Studies.Sci Rep, vol. 10, no. 1, May 2020, p. 8133. Pubmed, doi:10.1038/s41598-020-65205-8.
Nelsen E, Hobson CM, Kern ME, Hsiao JP, O’Brien Iii ET, Watanabe T, Condon BM, Boyce M, Grinstein S, Hahn KM, Falvo MR, Superfine R. Combined Atomic Force Microscope and Volumetric Light Sheet System for Correlative Force and Fluorescence Mechanobiology Studies. Sci Rep. 2020 May 18;10(1):8133.

Published In

Sci Rep

DOI

EISSN

2045-2322

Publication Date

May 18, 2020

Volume

10

Issue

1

Start / End Page

8133

Location

England

Related Subject Headings

  • RAW 264.7 Cells
  • Phagocytosis
  • Microscopy, Fluorescence
  • Microscopy, Atomic Force
  • Mice
  • Macrophages
  • Humans
  • Hela Cells
  • HeLa Cells
  • Fluorescence