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Comparison of liquid-based to tissue-based biopsy analysis by targeted next generation sequencing in advanced non-small cell lung cancer: a comprehensive systematic review.

Publication ,  Journal Article
Esagian, SM; Grigoriadou, GΙ; Nikas, IP; Boikou, V; Sadow, PM; Won, J-K; Economopoulos, KP
Published in: Journal of cancer research and clinical oncology
August 2020

To explore whether targeted next generation sequencing (NGS) of liquid biopsy in advanced non-small cell lung cancer (NSCLC) could potentially overcome the innate problems that arise with standard tissue biopsy, like intratumoral heterogeneity and the inability to obtain adequate samples for analysis.The Scopus, Cochrane Library, and MEDLINE (via PubMed) databases were searched for studies with matched tissue and liquid biopsies from advanced NSCLC patients, analyzed with targeted NGS. The number of mutations detected in tissue biopsy only, liquid biopsy only, or both was assessed and the positive percent agreement (PPA) of the two methods was calculated for every clinically relevant gene.A total of 644 unique relevant articles were retrieved and data were extracted from 38 studies fulfilling the inclusion criteria. The sample size was composed of 2000 mutations tested in matched tissue and liquid biopsies derived from 1141 patients. No studies analyzed circulating tumor cells. The calculated PPA rates were 53.6% (45/84) for ALK, 53.9% (14/26) for BRAF, 56.5% (13/23) for ERBB2, 67.8% (428/631) for EGFR, 64.2% (122/190) for KRAS, 58.6% (17/29) for MET, 54.6% (12/22) for RET, and 53.3% (8/15) for ROS1. We additionally recorded data for 65 genes that are not recommended by current guidelines for mutational testing. An extra category containing results of unspecified genes was added, with a PPA rate of 55.7% (122/219).Despite many advantages, liquid biopsy might be unable to fully substitute its tissue counterpart in detecting clinically relevant mutations in advanced NSCLC patients. However, it may serve as a helpful tool when making therapeutic decisions. More studies are needed to evaluate its role in everyday clinical practice.

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Published In

Journal of cancer research and clinical oncology

DOI

EISSN

1432-1335

ISSN

0171-5216

Publication Date

August 2020

Volume

146

Issue

8

Start / End Page

2051 / 2066

Related Subject Headings

  • Oncology & Carcinogenesis
  • Neoplastic Cells, Circulating
  • Lung Neoplasms
  • Liquid Biopsy
  • Humans
  • High-Throughput Nucleotide Sequencing
  • DNA Mutational Analysis
  • Circulating Tumor DNA
  • Carcinoma, Non-Small-Cell Lung
  • Biopsy
 

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Esagian, S. M., Grigoriadou, G. Ι., Nikas, I. P., Boikou, V., Sadow, P. M., Won, J.-K., & Economopoulos, K. P. (2020). Comparison of liquid-based to tissue-based biopsy analysis by targeted next generation sequencing in advanced non-small cell lung cancer: a comprehensive systematic review. Journal of Cancer Research and Clinical Oncology, 146(8), 2051–2066. https://doi.org/10.1007/s00432-020-03267-x
Esagian, Stepan M., Georgia Ι. Grigoriadou, Ilias P. Nikas, Vasileios Boikou, Peter M. Sadow, Jae-Kyung Won, and Konstantinos P. Economopoulos. “Comparison of liquid-based to tissue-based biopsy analysis by targeted next generation sequencing in advanced non-small cell lung cancer: a comprehensive systematic review.Journal of Cancer Research and Clinical Oncology 146, no. 8 (August 2020): 2051–66. https://doi.org/10.1007/s00432-020-03267-x.
Esagian SM, Grigoriadou GΙ, Nikas IP, Boikou V, Sadow PM, Won J-K, et al. Comparison of liquid-based to tissue-based biopsy analysis by targeted next generation sequencing in advanced non-small cell lung cancer: a comprehensive systematic review. Journal of cancer research and clinical oncology. 2020 Aug;146(8):2051–66.
Esagian, Stepan M., et al. “Comparison of liquid-based to tissue-based biopsy analysis by targeted next generation sequencing in advanced non-small cell lung cancer: a comprehensive systematic review.Journal of Cancer Research and Clinical Oncology, vol. 146, no. 8, Aug. 2020, pp. 2051–66. Epmc, doi:10.1007/s00432-020-03267-x.
Esagian SM, Grigoriadou GΙ, Nikas IP, Boikou V, Sadow PM, Won J-K, Economopoulos KP. Comparison of liquid-based to tissue-based biopsy analysis by targeted next generation sequencing in advanced non-small cell lung cancer: a comprehensive systematic review. Journal of cancer research and clinical oncology. 2020 Aug;146(8):2051–2066.
Journal cover image

Published In

Journal of cancer research and clinical oncology

DOI

EISSN

1432-1335

ISSN

0171-5216

Publication Date

August 2020

Volume

146

Issue

8

Start / End Page

2051 / 2066

Related Subject Headings

  • Oncology & Carcinogenesis
  • Neoplastic Cells, Circulating
  • Lung Neoplasms
  • Liquid Biopsy
  • Humans
  • High-Throughput Nucleotide Sequencing
  • DNA Mutational Analysis
  • Circulating Tumor DNA
  • Carcinoma, Non-Small-Cell Lung
  • Biopsy