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Gene assembly and combinatorial libraries in S. cerevisiae via reiterative recombination.

Publication ,  Journal Article
Ostrov, N; Wingler, LM; Cornish, VW
Published in: Methods Mol Biol
2013

While mutagenesis of single genes is now common practice in molecular biology, engineering multiple target genes still requires complex cloning techniques and thus is limited to expert laboratories. Here, we describe "Reiterative Recombination," a user-friendly DNA assembly technique in Saccharomyces cerevisiae for the integration of an indefinite number of DNA fragments sequentially into the yeast genome. The high efficiency of chromosomal integration can further be utilized for the assembly of large combinatorial libraries for metabolic engineering.

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Published In

Methods Mol Biol

DOI

EISSN

1940-6029

Publication Date

2013

Volume

978

Start / End Page

187 / 203

Location

United States

Related Subject Headings

  • Saccharomyces cerevisiae
  • Recombination, Genetic
  • Metabolic Engineering
  • Genetic Engineering
  • Developmental Biology
  • Cloning, Molecular
  • 3404 Medicinal and biomolecular chemistry
  • 3101 Biochemistry and cell biology
  • 0601 Biochemistry and Cell Biology
  • 0399 Other Chemical Sciences
 

Citation

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Ostrov, N., Wingler, L. M., & Cornish, V. W. (2013). Gene assembly and combinatorial libraries in S. cerevisiae via reiterative recombination. Methods Mol Biol, 978, 187–203. https://doi.org/10.1007/978-1-62703-293-3_14
Ostrov, Nili, Laura M. Wingler, and Virginia W. Cornish. “Gene assembly and combinatorial libraries in S. cerevisiae via reiterative recombination.Methods Mol Biol 978 (2013): 187–203. https://doi.org/10.1007/978-1-62703-293-3_14.
Ostrov N, Wingler LM, Cornish VW. Gene assembly and combinatorial libraries in S. cerevisiae via reiterative recombination. Methods Mol Biol. 2013;978:187–203.
Ostrov, Nili, et al. “Gene assembly and combinatorial libraries in S. cerevisiae via reiterative recombination.Methods Mol Biol, vol. 978, 2013, pp. 187–203. Pubmed, doi:10.1007/978-1-62703-293-3_14.
Ostrov N, Wingler LM, Cornish VW. Gene assembly and combinatorial libraries in S. cerevisiae via reiterative recombination. Methods Mol Biol. 2013;978:187–203.

Published In

Methods Mol Biol

DOI

EISSN

1940-6029

Publication Date

2013

Volume

978

Start / End Page

187 / 203

Location

United States

Related Subject Headings

  • Saccharomyces cerevisiae
  • Recombination, Genetic
  • Metabolic Engineering
  • Genetic Engineering
  • Developmental Biology
  • Cloning, Molecular
  • 3404 Medicinal and biomolecular chemistry
  • 3101 Biochemistry and cell biology
  • 0601 Biochemistry and Cell Biology
  • 0399 Other Chemical Sciences