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Cold sensitivity of the SARS-CoV-2 spike ectodomain.

Publication ,  Journal Article
Edwards, RJ; Mansouri, K; Stalls, V; Manne, K; Watts, B; Parks, R; Janowska, K; Gobeil, SMC; Kopp, M; Li, D; Lu, X; Mu, Z; Deyton, M ...
Published in: bioRxiv
October 13, 2020

The SARS-CoV-2 spike (S) protein, a primary target for COVID-19 vaccine development, presents its Receptor Binding Domain in two conformations: receptor-accessible "up" or receptor-inaccessible "down" conformations. Here, we report that the commonly used stabilized S ectodomain construct "2P" is sensitive to cold temperature, and that this cold sensitivity is resolved in a "down" state stabilized spike. Our results will impact structural, functional and vaccine studies that use the SARS-CoV-2 S ectodomain.

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Published In

bioRxiv

DOI

Publication Date

October 13, 2020

Location

United States

Related Subject Headings

  • Developmental Biology
  • Biophysics
  • 11 Medical and Health Sciences
  • 06 Biological Sciences
  • 03 Chemical Sciences
 

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Edwards, R. J., Mansouri, K., Stalls, V., Manne, K., Watts, B., Parks, R., … Acharya, P. (2020). Cold sensitivity of the SARS-CoV-2 spike ectodomain. BioRxiv. https://doi.org/10.1101/2020.07.12.199588
Edwards, Robert J., Katayoun Mansouri, Victoria Stalls, Kartik Manne, Brian Watts, Rob Parks, Katarzyna Janowska, et al. “Cold sensitivity of the SARS-CoV-2 spike ectodomain.BioRxiv, October 13, 2020. https://doi.org/10.1101/2020.07.12.199588.
Edwards RJ, Mansouri K, Stalls V, Manne K, Watts B, Parks R, et al. Cold sensitivity of the SARS-CoV-2 spike ectodomain. bioRxiv. 2020 Oct 13;
Edwards, Robert J., et al. “Cold sensitivity of the SARS-CoV-2 spike ectodomain.BioRxiv, Oct. 2020. Pubmed, doi:10.1101/2020.07.12.199588.
Edwards RJ, Mansouri K, Stalls V, Manne K, Watts B, Parks R, Janowska K, Gobeil SMC, Kopp M, Li D, Lu X, Mu Z, Deyton M, Oguin TH, Sprenz J, Williams W, Saunders K, Montefiori D, Sempowski GD, Henderson R, Alam M, Haynes BF, Acharya P. Cold sensitivity of the SARS-CoV-2 spike ectodomain. bioRxiv. 2020 Oct 13;

Published In

bioRxiv

DOI

Publication Date

October 13, 2020

Location

United States

Related Subject Headings

  • Developmental Biology
  • Biophysics
  • 11 Medical and Health Sciences
  • 06 Biological Sciences
  • 03 Chemical Sciences