Induction of C-MET mRNA in pancreatic cell lines in serum free condition by inflammatory cytokines

It is well established that the c- met oncogene encodes a protein receptor of i90KDa (hepatocyte growth factor receptor) which transmits multifunctional signals such as regulation of cell proliferation, motility and morphogenesis. Moghul e_t aj (1 ) described the effect of various inflammatory cytokines on c- met mRNA expression m human carcinoma cell lines. We have examined three pancreatic tumor ceJI lines BXPC3, SU8686, and PANC1 which show highly to poorly differentiated patterns of growth as xenograft tumors In this present study we used these three cell lines cultured without serum for 4,12, 24, and 48h at 37 C. Cells grew without any morphological changes until 24h and started dying afterwards. Initially, cells were conditioned with serum for i6h after plating then washed thoroughly with serum- free medium and incubated in serum- free medium for 3h. Inflammatory recombinant cytokines IL-1 and IL-6 at a dose of long/ml were added to 6 well plates and cells incubated for up to 24h then total RNA was extracted. At various time points cells were harvested, washed, and processed for total RNA extraction. Northern blot analysis showed that both the cytokines induced c-met mRNA under serum free condition. Therefore this study presents the first demonstration of the cytokine induced c-met mRNA in pancreatic tumor cell lines.These data suggest that inflammatory cytokines might interfere with cellular growth by altering the expression of c-met oncogene under serum free conditions.

Duke Scholars

Author William Clark Meyers Surgery